Publication: Detection of 9-bp deletion in COII/tRNALys intergenic region of mitochondrial DNA among murut ethnic group
| dc.contributor.author | Najah Farahin Nadiah Azman | |
| dc.date.accessioned | 2025-10-28T07:10:22Z | |
| dc.date.available | 2025-10-28T07:10:22Z | |
| dc.date.issued | 2025-02 | |
| dc.description.abstract | The 9-base-pair (bp) deletion in the COII/tRNALys intergenic region of the mitochondrial DNA (mtDNA) has been an important marker for haplogroup B classification in population group studies especially in Southeast Asia. However, no genetic data from 9-bp deletion was available for indigenous groups in Sabah including the Murut ethnic group due to the lack of population genetic studies done in these groups. The objective of this research is to fill in the research gap by investigating the presence or absence of 9-bp deletion of the COII/tRNALys intergenic region of mtDNA among the Murut ethnic group. A total of 100 unrelated Murut individuals participated in this research and were PCR amplified using a specific primer to generate 211 bp for absence of 9-bp deletion and a DNA band at 202 bp indicates the presence of 9-bp deletion. The finding shows 45% of Murut individuals were identified with 9-bp deletion and 55% were absence of 9-bp deletion. This outcome suggests that the Murut individual shows a considerably higher percentage of 9-bp deletion than other populations such as Papua New Guinea and Malaysian Han. In conclusion, typing of the COII/tRNALys intergenic region was able to provide information about the haplogroup structure among Murut individuals because the deletion at this region was used to confirm for haplogroup B | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/22975 | |
| dc.language.iso | en | |
| dc.subject | - | |
| dc.title | Detection of 9-bp deletion in COII/tRNALys intergenic region of mitochondrial DNA among murut ethnic group | |
| dc.type | Resource Types::text::thesis::bachelor thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |