Publication:
Innovative superhydrophobic platforms for growth enhancement in duckweed cultivation

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Date
2024-09-01
Authors
Chua, Mei Xia
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Duckweed, a fast-growing aquatic plant with rich nutritional content, contributes significantly to various fields like animal feed and bioenergy production. However, conventional cultivation methods such as ponds and tanks, encounter challenges water loss, nutrient imbalances, and scalability, limiting biomass yield and biochemical compositions. These challenges highlighted the need for efficient and scalable cultivation systems. Hence, this study addresses these challenges by developing a versatile platform through a novel approach of employing superhydrophobic properties to duckweed cultivation system. A robust beeswax superhydrophobic coating (156.06 °) was created by optimizing wax concentration and simplifying preparation of omitting sonication and annealing. The integration of this coating into cultivation platforms increased surface area and minimized water footprint for plant growth. To evaluate the performance of the superhydrophobic platform, duckweed (Lemna minor and Spirodela polyrhiza) was cultivated and compared with conventional platform of glass jar that served as the control. The findings demonstrated that the superhydrophobic platforms significantly improved the biomass production compared to control. L. minor showed superior growth in the superhydrophobic coated acrylic platforms (SHPA), while S. polyrhiza thrived best on the variant of the acrylic platform added with metal mesh (SHPAM). This work demonstrated the effectiveness of superhydrophobic coatings in promoting duckweed cultivation. Impacts of geometrical design of cultivation platforms in terms of efficient distribution of light and nutrient to duckweed were investigated. L. minor and S. polyrhiza were grown on platforms of different geometrical designs, including geometries of rectangle (SHP-R), oval (SHP-O) and circle (SHP-C). Both species showed the best performance of growth, nutrient uptake and biochemical quality in SHP-O. Additionally, a multi-tier cultivation system incorporating superhydrophobic coatings was investigated. The system (SHP-2T) utilized two tiers to enhance space utilization and improve light and nutrient distribution. The growth performance and nutrient uptake of L. minor and S. polyrhiza cultivated in respective tiers were evaluated. Dye-tracking and CFD simulation demonstrated an even flow pattern in SHP-2T system, ensuring efficient nutrient distribution, leading to significant reductions in ammonia and phosphate in the medium for both duckweed species. Both duckweed species displayed good growth rates and biochemical quality within the SHP-2T system, with no significant differences observed between tiers, validating the system's consistency. The successful development of superhydrophobic coatings, coupled with optimized platform design, has the potential to revolutionize duckweed production and unlock its full potential for various applications.
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