Publication: Production Of Monoclonal Antibodies Against Activin A Receptor Type Ii Like Kinase 1 (Alk-1) By Phage Display
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Date
2025-03
Authors
Nur Alia, Nur Alia
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Abstract
Atherosclerosis, a chronic inflammatory condition affecting the arterial wall, is a leading contributor to cardiovascular diseases (cvds). Low-density lipoprotein (ldl) is a key factor in the pathogenesis of atherosclerosis by promoting plaque formation within arteries. Activate a receptor type ii like kinase 1 (alk-1), a type i receptor of the transforming growth factor (tgf)-β superfamily, has been identified as a promising target for atherosclerosis treatment due to its role in the regulation of ldl levels. This study aimed to generate monoclonal antibodies (mabs) against alk- 1 using phage display technology for potential therapeutic applications. The alk-1 antigen was successfully expressed and purified, and its functionality was confirmed through binding assays with its ligand, bone morphogenetic protein-9 (bmp-9), and ldl. Biopanning of a human naïve single-chain variable fragment (scfv) antibody phage library against alk-1 was performed, resulting in the isolation of three unique scfv clones 4e, 9f, and 7c. Sequence analysis demonstrated that these clones pertained to distinct vdj gene families and exhibited varying complementaritydetermining region (cdr) lengths. Bioinformatics tools were used to predict the solubility of the scfv clones, which were subsequently expressed and purified from e. Coli.
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Activin receptors