Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation

Publication:
Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation

dc.contributor.author	Rajan, Kirutika Selva
dc.date.accessioned	2023-08-21T08:30:21Z
dc.date.available	2023-08-21T08:30:21Z
dc.date.issued	2022-03
dc.description.abstract	Ludisia discolor is a jewel orchid that is well known for the striking leaves than its flowers. Other than being used as an ornamental plant, this orchid is also being exploited for its medicinal properties. Hence, L. discolor facing possible depletion in its natural habitat lately due to over-harvesting. Recently, the latest cryopreservation methods such as V cryo-plate is needed for long-term germplasm conservation. This study aims to develop a V cryo-plate cryopreservation method for L. discolor axillary buds to perform microscopy analysis of cryopreserved explants, determine the water content changes, and genetic stability between control and treated explants. Six (6) parameters have been optimized in this V cryo-plate method which are preculture concentration and duration, types of loading solution, duration on PVS2, thawing temperature, and thawing duration.
dc.identifier.uri	https://erepo.usm.my/handle/123456789/17352
dc.subject	Development Of V Cryo-Plate Method Of Ludisia Discolor
dc.subject	For Long-Term Germplasm Conservation
dc.title	Development Of V Cryo-Plate Method Of Ludisia Discolor (Ker Gawl.) A. Rich For Long-Term Germplasm Conservation
dc.type	Resource Types::text::thesis::master thesis
dspace.entity.type	Publication
oairecerif.author.affiliation	Universiti Sains Malaysia

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