Publication: Changes in ph of digestive vacuole of plasmodium falciparum treated with pipecolisporin and analogue ii peptide
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Date
2025-01
Authors
Fauzi, Nurul Adila Ahmad
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Abstract
Malaria is a severe and fatal disease caused by Plasmodium spp. and remains one of the leading global causes of morbidity and mortality. The emergence of drug-resistant P. falciparum in various countries has necessitated an effort to discover new antimalarial drugs targeting different pathways. Research on the potential of pipecolisporin and analogue II as an antimalarial agent has remained limited. Therefore, this study aimed to fill this knowledge gap by investigating the antimalarial activity of pipecolisporin and analogue II peptides. The antimalarial potential of pipecolisporin and analogue II against the chloroquine-sensitive strain (3D7) of P. falciparum was assessed based on the calculation of parasitaemia using Giemsa stained-blood smears. The pipecolisporin and analogue II exhibited highly active antimalarial activity with an IC50 value of 0.4770 and 0.1170 μM, respectively. A further investigation focused on the effect of pipecolisporin and analogue II towards the pH of the mid trophozoite stage parasite’s digestive vacuole, employing a flow cytometry-based technique with fluorescein isothiocyanate-dextran (FITC-dextran) as a pH ratiometric probe. The results revealed no increase in pH following pipecolisporin and analogue II treatment. Suggests that pipecolisporin and analogue II might not have the mechanism to alter the digestive vacuole’s pH through the inhibition of V-type H+-ATPase that regulates the acidification of the vacuole. Overall, this study provides crucial evidence of pipecolisporin and analogue II capability as a promising antimalarial candidate
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