Publication: The role of hypoxia in controlling volatagegated sodium channels (VGSCS) induced breast cancer invasiveness
| dc.contributor.author | Makhtar, Noor Fatmawati | |
| dc.date.accessioned | 2023-12-06T08:27:05Z | |
| dc.date.available | 2023-12-06T08:27:05Z | |
| dc.date.issued | 2017 | |
| dc.description.abstract | This study was designed mainly to investigate the role of transcription factor, hypoxia inducible factor- 1a (HIF -1a) in enhancing Nav1.5 and nNav1.5 expression in breast cancer that transforms it to become aggressive. siRNA was conducted to knockdown HIF -1 a expression in the aggressive MDAMB- 231 cells whilst hypoxia-mimetic agent. cobalt chloride (CoCI 2) was used to stabilize HIF-1a in the less aggressive MCF-7 cells . Total RNA and protein were extracted and subjected to real-time PCR and Western blotting . Migration and motility assays were carried out to study effect of treatments (siRNA and CoCI2) on metastatic cell behaviours. Nav1 .5 , nNav1 .5, HIF-1a and CA9 expression were all hiqhlv expressed in MDA-MB-231 cells . siRNA caused a siqnificant decreased in HIF -1 a expression (mRNA and protein) and HIF-1 a-common target gene. CA9. mRNA expression of Nav1 .5 but not nNav1.5 was significantly downregulated by siRNA-HIF-1a followed by suppression of migration. When MCF-7 cells were treated with CoCI2 , HIF-1a protein, CA9 and Nav1.5mRNA expression was increased significantly but not nNav1 .5 followed by enhanced motility and migration. In conclusion. transcription factor, HIF-1a able to regulate Nav1 .5 (but not nNav1.5) in breast cancer to promote its aggressiveness. | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/17875 | |
| dc.language.iso | en | |
| dc.title | The role of hypoxia in controlling volatagegated sodium channels (VGSCS) induced breast cancer invasiveness | |
| dc.type | Resource Types::text::report | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |