Publication: The expression of HPV16/18 E6 and E7 oncoproteins in cervical lesions and the development of aptamer targeting HPV16 E6 oncoprotein
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Date
2022
Authors
Sabri, Nadiah Ahmad
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Abstract
Background: High risk human papilloma virus (HPV) particularly HPV16 and HPV18 had been the hallmark causative agent of cervical malignancy, in which overexpression of oncoprotein E6 and E7 induced the pathogenicity. The present study focused on evaluation HPV oncoproteins expression in formalin fixed paraffin embedded (FFPE) tissue in different grade of cervical lesions utilizing antibodies and in-silico designed DNA-Aptamer, as detection probe. Methods: A total of 102 FFPE cervical tissues of different grades were collected from Hospital Universiti Sains Malaysia (HUSM) and Hospital Raja Perempuan Zainab II (HRPZII) from the year of 2014 until 2022. Immunohistochemical analysis of E6 and E7 oncoproteins of HPV16 and HPV18 were evaluated. Novel DNA-Aptamer targeting HPV16-E6 oncoprotein were in-silico designed and characterized as alternative bioreceptor using histochemical staining application. Results: Increased expression of HPV18-E7 was seen with the progression of cervical lesions from the low-grade precursor to malignancy. Meanwhile, the HPV16-E7 expression was significantly associated with histological grade of HPV16-DNA confirmed positive cervical lesion (p=0.001). Aptahistochemistry using DNA-Aptamer targeting HPV16-E6 resulted in nuclear-specific staining in malignant cases with negligible non-specific background staining compared to the antibody-based immunohistochemistry. Conclusion: Immunohistochemical expression of HPV oncoproteins is a powerful alternative diagnostic tool, applicable in low-resource laboratory setting compared to the gold standard molecular testing. Meanwhile the DNA-Aptamer showed promising potential in the histochemical application with superior specificity than the immunohistochemical detection.
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Keywords
HPV16 , immunohistochemistry