Publication: Effects Of Pretreatments On Chemical And Physical Alterations Of Oil Palm (Elaeis Guineensis) Empty Fruit Bunch For Enhancing Enzymatic Saccharification Yield
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Date
2024-08
Authors
Tannimalay, Hemashini
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Abstract
The direct impacts of chemical composition and physicochemical properties alterations in pretreated biomass on the effectiveness of enzymatic saccharification yield have not been comprehensively elucidated. Therefore, this study investigates the effects of lignocellulose chemical composition and physicochemical properties of pretreated oil palm empty fruit bunch (OPEFB) fibre on enzymatic saccharification (total glucose yield, TGY). Three simple aqueous pretreatments (autohydrolysis, acid pretreatment and alkaline pretreatment) and mechanical refining were applied on oil palm empty fruit bunch (Elaeis guineensis) (OPEFB) fibres to enhance its enzymatic hydrolysability by the cellulolytic enzyme. The aqueous pretreatments were conducted at appropriate temperatures for 45 minutes with a solid: liquid ratio of 1:7. The refined untreated and pretreated OPEFB fibres were enzymatically saccharified at a fixed condition of 50°C and pH 4.8 for a duration of 48 hours with a substrate concentration of 25 g/L and an enzyme dosage of 70 FPU/g substrate of Celluclast 1.5 L. The results found that conducting aqueous pretreatment on unrefined OPEFB fibres caused lower lignocellulose degradation and dissolution than the pre-refined one, resulted in minimum cellulose loss and higher TGY. Based on the chemical composition analysis of OPEFB fibres and their enzymatic saccharification/ total glucose yield (TGY) by HPLC, autohydrolysed OPEFB fibre achieved the maximum (> 99.9%) TGY, attributed to 77% hemicellulose and 24% lignin removals, with only 16% cellulose loss during pretreatment.
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Keywords
Effects Of Pretreatments On Chemical , Physical Alterations , Physical Alterations Of Oil Palm (Elaeis Guineensis) , Empty Fruit Bunch , Enhancing Enzymatic Saccharification Yield , Tannimalay , Hemashini , Pusat Pengajian Teknologi Industri