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Development Of Tcr-like Single Domain Antibody By Incorporating Immunoglobulin Isotypes For Latent Tuberculosis Diagnostics And Therapeutics Application Against Heat Shock Protein 16 Kda Peptide Presented By Hla-a2

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dc.contributor.authorLiu, Hua Qiang
dc.date.accessioned2025-04-29T03:28:27Z
dc.date.available2025-04-29T03:28:27Z
dc.date.issued2024-08
dc.description.abstractHeat shock protein 16 kDa (HSP 16 kDa) is essential for the survival of latent Mycobacterium tuberculosis. Therefore, a peptide-MHC presentation of HSP 16 kDa could be a potential diagnostic and therapeutic target for latent tuberculosis (LTB). This study aimed to generate different immunoglobulin isotype pairings on a TCR-like single-domain antibody (sDAb) and subsequently investigate its diagnostic and therapeutic potential in LTB, utilizing a model cell presenting the target peptide. A previously generated TCR-like sDAb that can bind to HSP 16 kDa was first fused to a human IgG1, IgG2, IgG4, IgA, IgD, IgE, and IgM Fc-receptor via a linker. The fusion product, sDAb-Fc, was expressed in HEK293-F cells and subsequently purified. Its diagnostic potential was investigated via cell-based ELISA utilizing MCF-7 cells peptide-pulsed with HSP 16 kDa peptides. Antibody-dependent cell-mediated cytotoxicity (ADCC) of MCF-7 cells was also conducted to investigate its therapeutic potential. Finally, TCR-like sDAb-IgG1, IgG2, and IgG4 were successfully produced transiently in HEK293 F cells and purified using protein A chromatography.
dc.identifier.urihttps://erepo.usm.my/handle/123456789/21461
dc.language.isoen_US
dc.subjectDevelopment Of Tcr-like Single Domain Antibody
dc.subjectIncorporating Immunoglobulin Isotypes
dc.subjectLatent Tuberculosis Diagnostics
dc.subjectTherapeutics Application Against Heat Shock Protein 16 Kda Peptide Presented
dc.subjectHla-a2
dc.subjectLiu
dc.subjectHua Qiang
dc.subjectUniversiti Sains Malaysia
dc.subjectInstitut Penyelidikan Perubatan Molekul
dc.subjectSingle Domain Antibody
dc.titleDevelopment Of Tcr-like Single Domain Antibody By Incorporating Immunoglobulin Isotypes For Latent Tuberculosis Diagnostics And Therapeutics Application Against Heat Shock Protein 16 Kda Peptide Presented By Hla-a2
dc.typeResource Types::text::thesis::doctoral thesis
dspace.entity.typePublication
oairecerif.author.affiliationUniversiti Sains Malaysia
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