Publication: Protein adsorption from stickwater samples using magnetized cellulose nanocrystals
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Date
2022-03-01
Authors
Mahamood, Siti Nur Asiah
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Abstract
Magnetic cellulose adsorbent has gained attention in advanced bio-based nanomaterials and its unique high affinity characteristics towards organic matter forwastewater treatment. The aim of this research is to functionalize a magnetic cellulose nanocrystals (MGCNC) as protein adsorbent. MGCNC has been synthesized via 2,2,6,6-Tetramethylpiperidyl-1-Oxyl (TEMPO) mediated oxidation and fabricated through electrostatic self-assembly approach. The oxidation conditions were investigated under different conditions such as pH, sodium hypochlorite (NaClO)dosage, reaction time and temperature and found that the optimum conditions are pH 10.5, 12 mmol/g, 9 hours and 40°C. It was found that the carboxyl groups contents were mainly affected by the NaClO dosage and process temperature could affect the generation of carboxyl groups. The result revealed that the optimized MGCNC exhibited high degree of oxidation (0.095) with 70% of crystallinity percentage. The application of MGCNC in protein recovery was investigated through the adsorption kinetic and isotherm modelling. Based on experimental data, the pseudo-second-order kinetic model is suitable to describe the adsorption process of MGCNC at various concentration of protein, with good relation at R2=0.9918. Finally, the proposed adsorbent showed both high maximum adsorption capacities of bovine albumin serum(BSA) and stickwater (SW), (BSA: 84.264 mg g-1; SW: 70.94 mg g-1) and best fit to a Langmuir isotherm model. The proposed adsorbent MGCNC have great potential in protein recovery from wastewater.