Publication:
Exploring The Use Of Mspe As Extraction Method For Analysis Of Urinary Dap Metabolities Using Lc-ms

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dc.contributor.authorAmir Shah Ruddin, Nurul Iffah
dc.date.accessioned2026-05-12T02:10:32Z
dc.date.available2026-05-12T02:10:32Z
dc.date.issued2023-09
dc.description.abstractOrganophosphate (OP) pesticides have been widely used in agricultural sector. Past studies had shown that urinary dialkyl phosphate (DAP) metabolites can be reduced through organic diet intervention. Our study was focusing on developing an analytical method to analyse DAP metabolites in investigating the effect of organic diet intervention on OP pesticide exposure in rural and urban population. Our objectives were to validate the current existing method using liquid chromatography and massspectrometry (LC-MS) with solid phase extraction (SPE) extraction in analysing DAP metabolites in urine and to explore magnetic solid phase extraction (MSPE) as an extraction method for DAP metabolites analyses in urine. Analyses of DAP metabolites were performed using LC-MS with an Agilent Poroshell 120 EC-C18, 3.0 x 150 mm, 2.7 µm column. Due to time and sample limitation, we chose to analyse only diethyl thiophosphate (DETP) in urine sample because of its better yield compared to others. Calibration curve was constructed by spiking the urine sample at different concentration in the beginning of sample preparation for both SPE and MSPE methods. From our study, it was found out that MSPE method produced a better result compared to SPE method. The linearity of MSPE method was R2 = 0.9818 while SPE method produced R2 = 0.9789. The limit of detection (LOD) and limit of quantification (LOQ) of DETP through MSPE method were 0.51 and 1.54 part per million (ppm) respectively while SPE method recorded LOD of 0.86 ppm and LOQ of 2.62 ppm. Optimization of extraction time in MSPE method showed that 5 minutes was the best extraction time. This experiment was partially validated due to time constraint and more research is needed to optimize and validate it. The use of MSPE, which is simpler and faster in sample preparation step can be a steppingstone for future research in analysing DAP metabolites in urine.
dc.identifier.urihttps://erepo.usm.my/handle/123456789/24187
dc.language.isoen
dc.subjectNurul Iffah
dc.subjectAmir Shah Ruddin
dc.subjectExploring The Use Of Mspe
dc.subjectExtraction Method For Analysis Of Urinary Dap Metabolities Using Lc-ms
dc.subjectClinical Medicine
dc.titleExploring The Use Of Mspe As Extraction Method For Analysis Of Urinary Dap Metabolities Using Lc-ms
dc.typeResource Types::text::thesis::master thesis
dspace.entity.typePublication
oairecerif.author.affiliationUniversiti Sains Malaysia
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