Publication: Micropropagation Of Australian Pink Finger Lime (Citrus Australasica Cv. Mia Rose)
Loading...
Date
2025-06
Authors
Stephanie Wong Qiao, Er
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
Citrus australasica, commonly known as australian finger lime or “citrus caviar”, is renowned for its distinctive appearance, flavour and culinary versatility. It offers significant health benefits including high levels of antioxidants, vitamin c and minerals. However, conventional propagation methods, such as grafting and cuttings, are inefficient and time-consuming, often difficult to meet growing demand. Seed propagation is slow and produce genetically variable offsprings due to monoembryonic nature. Micropropagation offers a viable alternative for the rapid multiplication of disease-free plants under controlled conditions, suitable for establishing farm-ready plant stocks. This study aims to develop a complete micropropagation protocol for the australian pink finger lime (c. Australasica cv. Mia rose). Nodal explants were cultured on half-strength murashige and skoog (ms) media supplemented with cytokinins, including 6-benzylaminopurine (bap), kinetin (kn) and meta-topolin (mt), as well as combination of bap with indole-3-acetic acid (iaa) for shoot regeneration. Explants were also cultured in 2.0 mg/l bap with silver nitrate (agno3) or silver thiosulphate (sts) to assess their effects on ethylene-induced leaf abscission. Root induction was performed on woody plant medium (wpm) supplemented with 1.0 g/l activated charcoal (ac) alone, and in combination with iaa, indole-3-butyric acid (iba) and 1-naphthaleneacetic acid (naa). Vermicompost tea (vt) was also tested with selected treatments (1.0 g/l ac, 2.0 mg/l iaa and 1.0 mg/l naa) to improve rooting.
Description
Keywords
Micropropagation Australian Finger (Citrus Australasica Rose)