Publication: Molecular characterization of ornamental fosh (peocilidae) species by pcr-rflp of mitochondrial dna 12s rRNA gene.
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Date
2015
Authors
Azam, Mohamad Nor Fatihin Abshar Mohd Nor
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Abstract
In this study four ornamental fish species from Peociliidae family namely Xiphophorus maculatus, Xiphophorus hellerii, Poecilia reticulate, Limia Vitatta and Poecilia shenops v/qtq used as a samples. Characterization of fish species was carried out by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) followed by digestion with endonucleases restriction enzymes. Three different enzymes were used such as Alu I, Dde I and Mse I. Based on result only Xiphophorus maculatus showed species specific profile after treated with Alu I enzyme. No species specific was seen in all fish species after treated with Mse I and Dde I.