Publication: The role of TLR-2 IN the production of TNF-a and IL-10 by macrophage infected with a recombinant BCG (rBCG) expressing the MSP-1COF Plasmodium falciparum.
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Date
2015
Authors
Rajendran, Sasirekha D/O
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Abstract
An attenuated strain of Mycobacterium bow's BCG is the only available vaccine used for tuberculosis so far. The presence of glycolipids such as lipoarabinomannan (LAM) and lipomannan (LM) in its cell wall has encouraged the use of BCG as a recombinant vaccine vector for other pathogens, including malaria parasites. The benefits of using BCG as a recombinant vaccine vector include its ability to be ingested by professional antigen presenting cells (APCs) such as macrophage to induce pro-inflammatory responses, an important innate host defense mechanism against malaria infection. The interaction between BCG and macrophage involves several toll like receptors (TLRs) such as TLR-2. Therefore, this study was conducted to determine the role of TLR-2 in the production of inflammatory cytokines such as TNF-a and IL-10 by murine macrophage cell line, .I774A.1 infected with BCG and recombinant BCG (rBCG) clones expressing the MSP-1C of Plasmodium falciparum. The secretion of TNF-a and IL-10 cytokines by the infected macrophages in the absence or presence of TLR-2 was determined in the supernatant of the infected macrophages by enzyme-linked immunosorbent assay (ELISA). Our result showed that, higher levels of TNF-a and IL-10 were detected in the supernatant of BCG and rBCG infected macrophages either in the absence or presence of TLR-2 inhibitor. However, the levels of TNF-a and IL-10 cytokine production by infected macrophages in the absence of TLR-2 was significantly higher than macrophages stimulated with TLR-2. In conclusion, blocking of TLR-2 does not reduce the TNF-a and IL-10 production by the macrophages. Therefore, this result suggested that TLR-2 does not play important role in stimulating the production of TNF-a and IL-10 by macrophages infected with either BCG or rBCG clone.