Publication: Detection of synovial fluid-derived exosomes internalization by thp-1 cells
| dc.contributor.author | Faizal, Hanina Sofea | |
| dc.date.accessioned | 2025-11-27T02:15:25Z | |
| dc.date.available | 2025-11-27T02:15:25Z | |
| dc.date.issued | 2025-01 | |
| dc.description.abstract | Exosomes are small vesicles involved in intercellular communication, carrying bioactive molecules that influence immune responses and inflammation. Synovial fluid-derived exosomes have garnered attention for their potential roles in joint diseases like osteoarthritis and rheumatoid arthritis. Despite the growing interest in synovial fluidderived exosomes and their potential role in immune modulation and joint diseases, there is limited understanding of how these exosomes are internalized by immune cells, particularly monocytes, which this study aims to address by examining the internalization process in a human monocytic cell line (THP-1 cells). This study explores the internalization of these exosomes by THP-1 cells to understand their impact on immune modulation. DAPI-labelled THP-1 cells were cultured with PKH67-labelled synovial fluid-derived exosomes, and their interactions were observed using fluorescence microscopy. The uptake was analysed at two incubation times (24 and 48 hours) and cellto- exosome ratios (1:10, 1:20, and 1:40) using ImageJ software. Flow cytometry confirmed CD14 expression on THP-1 cells. Results showed significant exosome internalization, with the cell-to-exosome ratio being the key factor affecting uptake, while no significant difference was observed between the two incubation periods. This study emphasizes the role of exosome-to-cell ratio in the internalization process, contributing to the understanding of understanding of how these exosomes interact with immune cells | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/23178 | |
| dc.language.iso | en | |
| dc.subject | - | |
| dc.title | Detection of synovial fluid-derived exosomes internalization by thp-1 cells | |
| dc.type | Resource Types::text::thesis::bachelor thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |