Publication: The dynamical behavior of micro algae chlamydomonas reinhardtii before and after cargo picking
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Date
2022-01-01
Authors
Teng, Xiau Jeong
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Abstract
Interest in studying microbots has emerged due to seemingly promising application in the medical field. However, the lack of study on the change intransport behavior upon attachment of cargo is crucial from the engineering perspective. This work provided insightful study of dynamical behavior of the biohybrid microbot which allows possible engineering of microbot control in the future. Change in transport behavior of microalgae Chlamydomonas reinhardtii before and after picking up 4.5 μm polystyrene microbead as cargo was observed, recorded and analyzed. The microscopy of the free-swimming, collision into thecargo and swimming behavior after the attachment of the cargo was carried out by an Olympus BX53 microscope and recorded at 15 fps. The attachment of microbeadonto the microalgae by electrostatic interaction was confirmed by the change in surface electrophoretic mobility and non-attachment collision events. The microswimmer changed from linear forward motion into helical motion upon the attachment of the cargo and swam with a decreased swimming velocity. The underlying change upon the attachment of the cargo was revealed. The attachment of the microbead at the anterior end between the flagella bias towards either of the flagellum, causing imbalance torque and centripetal force. Meanwhile, 2 beadsattached microswimmer with reduced translational ability furthered confirmed the suppression of flagellum by the cargo attachment. The possibility of increased inviscosity drag was excluded by the trajectory of microswimmer with 1 posterior
attached microbead which exhibited translational ability similar to free-wimming cell. The experimental results and analysis showed that the loading location of the cargo
greatly influence the swimming behaviour of the microswimmer