Publication: Evaluation of bacterial and fungal surface contamination in the cell culture laboratory at the school of health sciences, University Sains Malaysia
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Date
2026-01
Authors
Azniff, Nur Ariena Faisol
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Abstract
Microbial contamination in laboratories, particularly on surfaces within cell culture
facilities, presents a significant challenge to maintaining culture quality and reliable
experimental outcomes. Understanding the types and sources of microbial contamination
is essential for improving laboratory hygiene and ensuring high-quality results. This study
aimed to quantify bacterial and fungal colony-forming units (CFUs) on selected hightouch
surfaces at different times of the day and to identify the predominant microbial
species present in a cell culture laboratory. Direct surface sampling was conducted, and
total heterotrophic plate counts were determined using nutrient agar and MacConkey agar
for bacteria, and Sabouraud dextrose agar for fungi. No significant differences were
observed in bacterial or fungal CFU counts between morning and evening sampling.
Overall, the highest microbial loads were detected on the water bath, followed by the
workbench. Staphylococcus spp., Bacillus spp., and Micrococcus spp. were the most
frequently isolated bacteria, while yeast spp., Rhizopus spp., Aspergillus spp., and Mucor
spp. were the most commonly detected fungi. These findings highlight the importance of
good laboratory practices, including aseptic techniques, routine cleaning, and proper use
of personal protective equipment (PPE). Further studies with larger sample sizes are
recommended to comprehensively characterise the microbial communities in cell culture
laboratories.