Publication: Anti-Diabetic Activity-Guided Studies Of Syzygium Polyanthum (Wight) Leaf Extracts And Elucidation Of Their Mechanisms Of Action
| dc.contributor.author | Widyawati, Tri | |
| dc.date.accessioned | 2025-10-02T07:26:10Z | |
| dc.date.available | 2025-10-02T07:26:10Z | |
| dc.date.issued | 2015-11 | |
| dc.description.abstract | Anti-diabetic activity guided studies were performed on the leaves of syzygium polyanthum, a popular traditional medicinal herb in north sumatera, indonesia. The anti-diabetic activity was evaluated by determining whether the extracts lowered the blood glucose levels (bgl) of normal rats (hypoglycaemic test), inhibited the rise of bgl of intraperitoneally glucose- loaded rats (glucose tolerance test) and lowered bgl of streptozotocin-induced diabetic rats. Dried powdered s. Polyanthum leaves were extracted serially with petroleum ether (pee), chloroform (ce), methanol (me) and water (we). In diabetic rats, single-dose administration of the extracts showed that only me reduced bgl. However, upon repeated-dose administration for 6 days both pee and me reduced bgl significantly. Therefore, liquid-liquid partition was used to fractionate me into the following fractions: chloroform (cf), ethyl acetate (eaf), n-butanol (n-bf) and water (wf). Repeated administration of cf and wf decreased bgl of diabetic rats. Cf was shaken with n-hexane to yield an undissolved fraction (sf-1) and a dissolved fraction (sf-2). Onlysf-1 lowered bgl of diabetic rats. Gc-ms identified the presence of squalene (sq)in me, cf and sf-1. Repeated administration of sq reduced bgl of diabetic rats.This suggests that sq is one of the compounds in s. Polyanthum leaf that contributeto its anti-diabetic activity. Repeated-dose for 12 days of me, cf, wf, sf-1 and sqproved that me was the most active blood glucose lowering agent. | |
| dc.identifier.uri | https://erepo.usm.my/handle/123456789/22687 | |
| dc.language.iso | en | |
| dc.subject | Anti-Diabetic Activity-Guided Studies | |
| dc.title | Anti-Diabetic Activity-Guided Studies Of Syzygium Polyanthum (Wight) Leaf Extracts And Elucidation Of Their Mechanisms Of Action | |
| dc.type | Resource Types::text::thesis::doctoral thesis | |
| dspace.entity.type | Publication | |
| oairecerif.author.affiliation | Universiti Sains Malaysia |