Elicitation Effect On The Growth And Biochemical Activities Of Curcuma Mangga Val (Zingiberaceae) In Vitro Plantlets

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dc.contributor.authorAbraham, Fariz
dc.date.accessioned2018-06-25T01:22:53Z
dc.date.available2018-06-25T01:22:53Z
dc.date.issued2010-12
dc.description.abstractCurcuma mangga aseptic plantlets could be established via double-stage surface sterilization technique. The bud explants derived from the rhizomes were immersed in 70% (v/v) alcohol for ten minutes at the first stage followed by surface-sterilized with 20% (v/v) Clorox® for 20 minutes at the second stage. The established aseptic explants were then cultured on the best proliferation medium, which is the MS medium supplemented with 2 mgL-1 6-benzylaminopurine (BAP) and 0.5 mgL-1 1-naphthaleneacetic acid (NAA). Liquid proliferation medium induced more auxiliary shoots (6.7 ± 0.4 shoots/explant) as compared to gelled proliferation medium (4.6 ± 0.6 shoots/explant). Quarterly divided shoot explants produced more shoots (7.6 shoots/ explant) compared to half divided and whole shoot explants. Mode of inoculation (vertically or horizontally) did not affect shoot production of C. mangga. Different pH of the culture medium (5.7, 7.0 and 8.0), illumination conditions (continuous illumination and total darkness), and concentration of yeast extract (0-5.0 mgL-1) added into the culture medium did not affect the plantlet biomass and number of new shoots produced from each explants. Growth retardation and chlorosis were often detected in plantlets cultured in alkaline proliferation medium (pH 8.0), high concentration of yeast extract (3.5 and 5.0 mgL-1), high concentration of chitosan (100-200 mgL-1), and the absence or higher strength than normal MS micronutrients strength (0 or 3-12 strength of MS micronutrients). Etiolated growth and chlorosis were obviously detected in plantlets cultured in total darkness condition. Other abnormal changes such as brittle petiole and inhibition of shoots and roots formation were only detected in plantlets cultured in medium supplemented with high concentration of chitosan (100-200 mgL-1) + 3.5 mgL-1 yeast extract. The presence of high concentration of chitosan (150 mgL-1) + 3.5 mgL-1 yeast extract significantly affected the new shoots production as compared to control. C. mangga plantlets could only tolerate the presence of half and full strength MS micronutrients in medium. Biomass (fresh and dried) of plantlets was affected by the presence of MS micronutrients higher than full strength or without the addition of MS micronutrients in medium but not the number of new shoots produced. Plantlets cultured in medium supplemented with yeast extract (3.5 and 5.0 mgL-1) and in medium supplemented with 150 mgL-1 chitosan + 3.5 mgL-1 yeast extract exhibited high and stable free radical scavenging activity from 30 to 130 minutes of incubation time.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/5765
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectCurcuma mangga aseptic plantlets could be establisheden_US
dc.subjectvia double-stage surface sterilization techniqueen_US
dc.titleElicitation Effect On The Growth And Biochemical Activities Of Curcuma Mangga Val (Zingiberaceae) In Vitro Plantletsen_US
dc.typeThesisen_US
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