HLA Genetic Profile Of Malay Sub-Ethnic Groups Of Peninsular Malaysia As Determined By Pcr-Ssp And Sbt Methods
| dc.contributor.author | Shahril, Allia | |
| dc.date.accessioned | 2017-09-08T06:57:47Z | |
| dc.date.available | 2017-09-08T06:57:47Z | |
| dc.date.issued | 2016-08 | |
| dc.description.abstract | The Indo-Malay Archipelago is the homeland of the Austronesian speaking population known as the Malays. Now, the definition of Malay is confine to the population residing in Malaysia. Due to the different origin of the Malays in Malaysia from various parts of the Malay Indo-Archipelogo, there are many Malay sub-ethnic groups living in Malaysia. The main objective of this research is to study the distribution of the HLA alleles and haplotypes in the Malay sub-ethnic groups. The human leukocyte antigen (HLA) class I and II allele distributions were investigated in 222 unrelated Malay individuals belonging to six Malay sub-ethnic groups namely Kelantan (64), Champa (42), Patani (30), Kedah (30), Aceh (30) and Mandailing (30) in Peninsular Malaysia. The HLA-A, -B, -C, -DQB1 and -DRB1 allele groups were typed using sequence specific primer (SSP) method. Further analysis was carried out using sequence-based typing (SBT) method on 120 individuals comprising of four Malay sub-ethnic groups namely Kelantan (30), Champa (30), Patani (30) and Mandailing (30) Malays to investigate the HLA-A, -B and -DRB1 allele distribution. For the HLA-SSP method, HLA-A*24, HLA-B*15, HLA-C*07, HLA-DQB1*03 and HLA-DRB1*15 are the most common allele groups for the five HLA loci examined in the combined Malay population (CMP). The most common HLA haplotypes in the CMP are HLA-A*02-C*08, HLA-C*08-B*15, HLA-DQB1*03-DRB1*12, HLA-A*02-C*08-B*15 and HLA-A*33-B*44-DRB1*07. For the HLA-SBT method, the most common HLA alleles in Kelantan, Champa, Patani and Mandailing respectively are; HLA-A*24:07, HLA-B*15:02 and HLA-DRB1*12:02; HLA-A*11:01, HLA-B*15:02 and HLA-DRB1*15:02; HLA-A*33:03, HLA-B*58:01 and HLA-DRB1*12:02; HLA-A*02:01, HLA-B*15:13 and HLA-DRB1*12:02. Based on the HLA distribution, Kelantan, Champa and Patani Malays bear similar HLA allele distribution.Mandailing Malays exhibits a slightly different pattern of HLA distribution and have close genetic relationship with the Filipinos based on their most frequent haplotypes. Overall, the Malay sub-ethnic groups share close genetic relationship with each other and also with other Austronesian speaking Southeast Asian population groups. This study forms a basic database for the research on population genetic, transplantation and disease association studies. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/4547 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Malay Sub-Ethnic Groups Of | en_US |
| dc.subject | Peninsular Malaysia. | en_US |
| dc.title | HLA Genetic Profile Of Malay Sub-Ethnic Groups Of Peninsular Malaysia As Determined By Pcr-Ssp And Sbt Methods | en_US |
| dc.type | Thesis | en_US |
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