Development Of Bacterial Culture For Odour Control
| dc.contributor.author | Chin, Siat Pei | |
| dc.date.accessioned | 2018-12-05T01:31:40Z | |
| dc.date.available | 2018-12-05T01:31:40Z | |
| dc.date.issued | 2011-08 | |
| dc.description.abstract | Volatile fatty acids particularly butyric acid is known as the major component of odour, associated with livestock farming, agricultural activities and wastewater treatment system. Thirteen butyric acid-degrading bacteria were isolated from the rivers and cow farm in Malaysia. Screening with specific growth rates and butyric acid degradation rates had selected four strains of highest values. These four bacteria were identified as Cupriavidus sp. strain B3, Burkholderia sp. strain C2, Acinetobacter sp. strain C4 and Cupriavidus sp. strain C5. Among the four, Acinetobacter sp. strain C4 showed the highest values of specific growth rate at (0.321±0.002) h-1, and degradation rate at 54 ppm butyric acid/h. Further studies revealed that Acinetobacter sp. strain C4 was able to perform butyric acid degradation at pH 6-9 and temperature of 25-40ºC. Additionally, this bacterium was found degrading other volatile fatty acids such as acetic acid, propionic acid, valeric acid and caproic acid, which were reported to deplete air quality extensively. In the cells production course, cultivation medium was formulated with 15.50 g/L yeast extract. Acinetobacter sp. strain C4 was cultivated through exponential feeding at 0.086 h-1 specific growth rate, and the cells concentration achieved was (8.729±0.352) g/L at 12 h of fed. A significant decline in growth thereafter was presumed to be correlated with the generation of inhibitory metabolites. Hence, continuous culture at different dilution rates (0.086, 0.20, 0.26, 0.35, 0.43 h-1) were implemented to eliminate substrate limitation and metabolites inhibition. The highest cells concentration, (5.206±0.050) g/L was obtained at 0.086 h-1, while the maximum cells productivity, (0.926±0.034) g/L/h was achieved at 0.20 h-1. The fermentation studies showed that continuous culture demonstrated the feasibility to achieve high cells productivity. The produced cells were then immobilized in polyvinyl alcohol and incorporated in the biofilter development. The biofilter was constructed with a glass column of 24 2 cm (height diameter) and packed with 24 g polyvinyl alcohol cubes containing 0.24 g dry cells Acinetobacter sp. strain C4. Under aerobic condition, the propionic acid (452±2 ppm), butyric acid (1371±4 ppm) and valeric acid (1399±4 ppm) in cow farm wastewater were removed at (63.4±2.2)%, (66.2±0.6)% and (61.2±1.1)%, respectively at 96 h. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/7194 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Development of bacterial culture | en_US |
| dc.subject | for odour control | en_US |
| dc.title | Development Of Bacterial Culture For Odour Control | en_US |
| dc.type | Thesis | en_US |
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