Typing of Burkholderia pseudomallei and association of its genotypes with clinical presentations and virulence
Loading...
Date
2015
Authors
Harun, Aziah
Journal Title
Journal ISSN
Volume Title
Publisher
Pusat Pengajian Sains Kesihatan, Universiti Sains Malaysia
Abstract
Burkhoderia pseudomallei is a soil dwelling Gram-negative bacteria found mainly in the Southeast Asia region and
northern Australia. Bacterial isolates from recent cases of melioidosis were collected and the genotypic diversity among
these was explored using multi-locus sequence typing (MLST). Massive genotypic heterogeneity was observed among
the 85 strains in which 23 different sequence profiles were observed. Sequence type 54 (ST54) was noted to
predominate and two novel sequence types were discovered. Most of sequence types in this study were genetically
related to each other and were clustered in a same group with those from Southeast Asian region and derived from the
same ancestor which was ST 48. Analysis of association between the genotypes and clinical presentations explored the
association of genotypes with predisposing factors, type of manifestation, severity of sepsis and mortality due to
melioidosis. With regard to virulence association with genotypes, survival study and tissue burden analysis have been
explored .
Description
Keywords
genotypes