Production Of Crude Biopolymer From Bacteria In Drainage System
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Date
2014-01
Authors
Abdul Talib, Azieda
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
This work focuses on the extraction of biopolymer from bacteria in relation to
wastewater treatment, specifically in flocculation process. Three isolates of bacterial
strain (WIR, WRA, WIA) which are able to produce biopolymers as flocculants were
identified from industrial and residential drainage systems. These bacteria were
identified as Bacillus megaterium BMRA, Bacillus megaterium BMIR and Bacillus
subtilis BSIA through physiological characteristics (mucoid, slimy and ropy) and the
partial sequences of their 16SrRNA. Crude biopolymers CWIR (industrial +
residential) and CWRA (residential) were produced by Bacillus megaterium BMIR
and BMRA while CWIA (industrial) was extracted from Bacillus subtilis BSIA.
These bacteria produced their respective biopolymers at 40ºC given a two-day
incubation period, with pH 7.0 ± 0.03. The crude biopolymers were recovered from
the supernatant of the culture broth by ethanol precipitation and vacuum-dried in
vacuum oven. CWIR, CWRA and CWIA contained 4.01%, 5.20% and 5.45% of
protein respectively. Total sugar content in each biopolymer was 1.48% for CWIR,
1.65% for CWRA and 1.79% for CWIA. Elemental analyses showed that the CWIR
obtained had 31.60% of C, 4.81% of H and 6.32% of N, while WRA contained
31.37% of C, 5.02% of H and 6.12% of N. WIA showed a composition comprising
31.25% of C, 4.88% of H and 5.69% of N. The molecular weight for each of the
biopolymers CWIA, CWIR and CWRA was measured at 31.788 kDa, 55.344 kDa and
38.791 kDa respectively.
Description
Keywords
Crude Biopolymer , Bacteria