A Preliminary Study On Platelet Concentrates Quality Collected From Two Different Mixing Methods: Manual Mixing Versus Automated Blood Collection Mixer

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Date
2017-06
Authors
Lim, Wen Siew
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Publisher
Universiti Sains Malaysia
Abstract
There are two different mixing methods used in whole blood collection which are manual mixing and automated blood collection mixer. The purpose of mixing collected blood with anticoagulant in primary bag is to prevent platelet and clotting factors early activation. Platelet concentrates quality might be affected due to inadequate mixing process during collection process. Therefore, this study is to evaluate whether manual mixing method produces similar quality of platelet concentrates with automated blood collection mixer. In this study, total 30 eligible participants were included. 15 units of whole blood were mixed by automated blood collection mixer and another 15 units were mixed by manual mixing method. Platelet concentrates were produced by using platelet rich plasma method. Platelet concentrates were stored at 20⁰C to 24⁰C, continuous agitation for 5 days. Quality control parameters such as total platelet count, total white blood cell and pH were tested on these platelet concentrates at Day 1 and Day 5. This study result showed all platelet concentrates achieved desired quality requirement from National Blood Centre Guideline with total white blood cell less than 0.2 x 109/unit and pH value more than 6.4. However, only 27% to 40% of the platelet concentrates met the national standard requirement in which the platelet counts were more than 60x109/unit. Total platelet count parameter showed significant median difference (p<0.05) from Day 1 to Day 5 for both types of mixing method. However, there were no statistical significant difference (p>0.05) for total white blood cell and pH from Day 1 and Day 5 for both types of mixing method. There was no statistically significant difference (p>0.05) between two mixing methods at both Day 1 and Day 5 for all the parameters measured. In conclusion, quality platelet concentrates which are total platelet count, total white blood cell and pH collected by manual mixing method has similar outcomes with automated blood collection mixer.
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Keywords
Platelet concentrates quality collected , manual mixing versus automated blood collection mixer
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