Measurement of oxygen uptake rate (OUR) during start-up phase of aerobic granular sludge (AGS) formation in leachate treatment

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Date
2019-06
Authors
Nurul Najihah Binti Ahmad
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Sanitary landfill is the most common method to dispose municipal solid waste due to such advantages as simplicity, low price and landscape-restoration of holes from mineral workings. Treating and managed safely landfill leachate is very important to avoid environment hazard to surface and ground water as it can percolate throughout soils and subsoils causing adverse impacts to receiving waters. Aerobic granular sludge (AGS) is one of the technologies that use activated sludge from Indah Water Konsortium (IWK) and it has a great potential in reducing footprint and improving efficiency of municipal and industrial wastewater treatment plants. AGS is a novel microbial community that allows simultaneous removal of carbon, nitrogen, phosphorus and other pollutants in a single sludge system. The AGS method provides unique characteristics, including compact structure, high settling velocity, ability to sustain high biomass concentration and simultaneous nutrient removal capability. On the other hand, oxygen uptake rate (OUR) defined as the rate at which microorganisms use oxygen is an indicator of the biological activity of the system. While for specific oxygen uptake rate (SOUR), it described the amount of oxygen used by the microorganisms to consume one gram of food. Both OUR and SOUR were used as the parameters to characterised the leachate during AGS formation. This research aims to cultivate AGS using leachate as a substrate. After 50 days operation, a small and compact sludge with size 25 - 163 µm were obtained. It decreases in size from 219 - 1612 µm on the first day operation, which has a flat, loose and irregular structure. The analysis on the relationship between OUR and AGS formation based on mixed liquor suspended solid (MLSS), mixed liquor volatile suspended solid (MLVSS), sludge retention time (SRT), food to microorganism (F/M) ratio and also removal effectively in suspended solid (SS) with maximum removal of 80%. While for chemical oxygen demand (COD), maximum removal of 67% is obtained. Next, F/M increased from 0.65 g COD/g MLVSS d on day 15 to 3.52 g COD/g MLVSS d on day 30. This is followed by simultaneous increment of SOUR on day 22 to day 30 which are 130 mg O2/g MLVSS h and 263 mg O2/g MLVSS h, respectively. It shows that SOUR increases when F/M increases. Due to a low organic loading rate (OLR), which was from 0.84 kg COD/m3d on day 12 to 0.62 kg COD/m3d on day 15 has resulting in lower MLSS to 0.72 g/L on day 22. The disintegration that occurs on day 12 resulted in lower SRT (4 days) reducing the OUR value (0.63 mg O2/ L min ) which should be it is higher than SOUR (177 mg O2/ g MLVSS h). Also on day 12, lower OUR is because of higher F/M ratio (3.77 g COD/g MLVSS d). This is due to lower consuming of oxygen by the lower microorganisms.
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