Modulation of activated macrophage immune response by Carica papaya leaf extract
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Date
2016-09
Authors
Zatil Aqma Mohd Noor
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Carica papaya is a plant known for its medicinal attributes in traditional
remedies. Previous usage revealed that C. papaya crude aqueous leaf extract exhibits
positive effects on immune cells; increased platelet, white blood cell and neutrophil
counts in dengue patients. However, the helpful effects of C. papaya crude aqueous
leaf extract have not been scrutinized for possible understanding of its immune
modulator activity. In this study, immune modulator activity of C. papaya crude
aqueous leaf extract was tested on activated macrophage. Cytokines activities,
specifically tumor necrosis factor alpha (TNF-a) was used as the indicator for
immune modulator effects on the activated macrophage. The presence of the extract
demonstrated decreasing amount of TNF-a detectable in cell culture supernatant. The
decreased in TNF-a amount by the activated macrophage was detected by ELISA,
suggesting for a reduction in TNF-a production. On the contrary, results from realtime
RT-PCR did not show suppression of mRNA TNF-a expression but rather a
noticeable activation of the TNF-a gene. Thus, it could be speculated that the extract
did not inhibit the production of TNF-(x and subsequently its secretion. Rather, the
extract could possibly interfere with the fiinctions 0f TNF-a.
Description
Keywords
Macrophages -- Activation , immune response