Development Of Thermostabilized Pcr Method For The Simultaneous Detection Of Snps Of Cyp2b6 And Oprm1 Genes

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Date
2011-02
Authors
Kc, Sanjaya
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Publisher
Universiti Sains Malaysia
Abstract
Illicit heroin use and HIV are double threats faced by Malaysia and some parts of the world. Malaysian AIDS Council reported the total number of HIV cases in Malaysia to be 86,127 by June2009, was merely 3 in the year 1986. With the rate of increase in HIV it is estimated that about 24 million Malaysians will be infected or affected by HIV/AIDS by the year 2020 where the total population is estimated to be 40 million. In Malaysia and parts of South East Asia, the prime vehicle for HIV transmission is the sharing of contaminated injection needles by drug users. Statistics shows that 71% of the HIV cases in Malaysia are due to sharing of needles/syringe by the Injecting drug users. To combat the threats, methadone and antiretrovirals are widely used. These drugs are influenced by CYP2B6 and for methadone, μ Opiate-receptor is also involved. Both are genetically polymorphic. Our objective was to develop a multiplexed allelespecific PCR for the simultaneous detection of these polymorphisms to optimize therapies with methadone and antiretrovirals and to freeze dry the PCR method so as to make it feasible for routine diagnostic laboratory use. Regions from μ Opiate-receptor parallel allele-specific second PCR. Three single nucleotide polymorphisms (SNPs) from CYP2B6, and three SNPs from OPRM1were targeted.
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Keywords
Thermostabilized Pcr Method , Cyp2b6 And Oprm1 Genes
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