Identification Of Selected Corticolous Lichens From Penang Hill, Gunung Jerai And Cameron Highlands And In Vitro Culture Of Their Mycobionts

Identification Of Selected Corticolous Lichens From Penang Hill, Gunung Jerai And Cameron Highlands And In Vitro Culture Of Their Mycobionts

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dc.contributor.author	CHRISTINE
dc.date.accessioned	2016-06-21T02:20:52Z
dc.date.available	2016-06-21T02:20:52Z
dc.date.issued	2015-07
dc.description.abstract	Lichens are symbiotic associations between algae and fungi. In Malaysia, studies on lichens are limited. Fifty three lichen species were collected from three different locations, namely Cameron Highland, Gunung Jerai and Penang Hill. Their morphological, anatomical and chemical characteristics were studied. Graphidastra multiformis, Coenogonium nepalense and Malmidea inflata were reported as new to Malaysia. Isolation of mycobionts from selected lichen species were successfully carried out by spore discharge technique. The optimum sucrose concentration for the growth of the mycobiont colonies of Graphis arbusculaeformis and Glyphis scyphulifera was 30 g/L. The optimum temperature for the growth of mycobionts of G. arbusculaeformis was 25°C and that of G. scyphulifera was 20-25°C. Highest biomass was obtained when the mycobionts of G. arbusculaeformis cultured in MY30 medium [1.36 ± 0.08 g (fresh) and 219.0 ± 11.07 mg (dried)] and potato dextrose agar medium supplemented with 30 g/L sucrose (PD30) [1.41 ± 0.11 g (fresh) and 232.14 ± 9.63 mg (dried)]. When the mycobionts of G. scyphulifera were cultured in different media high biomass could be obtained on MY30 [2.10 ± 0.15 g (fresh) and 375.71 ± 36.32 mg (dried)], PD30 [1.89 ± 0.11 g (fresh) and 369.42 ± 9.31 mg (dried)] and Sabouraud agar medium (SAM) [2.03 ± 0.13 g (fresh) and 368.28 ± 16.55 mg (dried)]. Mycobionts of G. arbusculaeformis and G. scyphulifera produced significantly higher biomass when cultured under agitated condition than those cultured under stationary mode. The fresh and dried biomasses of mycobionts of G. arbusculaeformis cultured under agitated condition were 3.14 ± 0.14 g and 296.43 ± 19.74 mg respectively and those of G. scyphulifera were 7.07 ± 0.31 g and 567.0 ± 26.93 mg respectively. When the G. arbusculaeformis mycobionts were subcultured continuously in liquid MY 30 medium it showed decrease in biomass. However, the mycobionts of G. scyphulifera showed increase in biomass in the first subculture and the biomass decreased at the end of the sixth subculture cycle. The suitable initial pH of the medium for the culture of mycobionts of G. arbusculaeformis and G. scyphulifera was from pH 4 to 7 and pH 5 to 7 respectively. Glucose and sucrose promoted the growth of mycobionts as compared to fructose and maltose. When cultured on solid MY30 medium, mycobionts of Sarcographa labyrinthica produced red pigmented colonies. Acetone extracts of S. labyrinthica showed antimicrobial activity against Bacillus subtilis (MIC-2500 μg/mL), Escherichia coli (MIC-2500 μg/mL) and Pseudomonas aeruginosa (MIC-1250 μg/mL)	en_US
dc.identifier.uri	http://hdl.handle.net/123456789/2148
dc.subject	Identification Of Selected Corticolous Lichens From Penang Hill, Gunung Jerai And Cameron Highlands	en_US
dc.subject	And In Vitro Culture Of Their Mycobionts	en_US
dc.title	Identification Of Selected Corticolous Lichens From Penang Hill, Gunung Jerai And Cameron Highlands And In Vitro Culture Of Their Mycobionts	en_US
dc.type	Thesis	en_US

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