Development And Evaluation Of An Antigen Detection Test For Hydatid Cyst Disease

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dc.contributor.authorKhanbabaie, Sam
dc.date.accessioned2019-04-17T06:38:02Z
dc.date.available2019-04-17T06:38:02Z
dc.date.issued2018-05
dc.description.abstractHydatid cyst disease (HCD) is a zoonotic parasitic disease which can infect both humans and animals. Imaging and antibody detection tests have been used to diagnose HCD, the former cannot differentiate between hydatid cyst and other pathologies; while the latter may not be able to differentiate between past and present infections, and may also not be suitable for evaluating treatment efficacy. In this regard, antigen detection tests may be able to assist in addressing the above issues. Previously it has been reported that hydatid cyst antigens can be detected in HCD patients using various assays. However the reported results were not satisfactory, with variable diagnostic sensitivities (25% - 83%). Therefore, this study was aimed at developing an antigen detection test for HCD. A panel of samples comprising serum from HCD patients (n=35), healthy individuals (n=38) and patients with other parasitic diseases (n=13) were used. Three types of E. granulosus antigens were prepared i.e. hydatid cyst fluid (HCF), native antigen B (nAgB) and recombinant antigen B (rAgB). Polyclonal antibodies against each of the antigens were produced in rabbits, followed by purification of their IgG fractions. Next, a portion of the purified IgGs were labelled with horseradish peroxidase (HRP). Using the three types of IgGs and three types of HRP-conjugated antibodies, various ELISA sandwich combinations were tested. The best ELISA combination was further optimized and evaluated using the panel of serum samples. Another portion of the purified IgGs were conjugated to colloidal gold nanoparticles for development of lateral flow dipstick (LFD). The dipsticks were dotted with three different IgGs, and LFD assay using various combinations of capture and detector antibodies were tested.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8028
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectEvaluation of an antigen detectionen_US
dc.subjecttest for hydatid cyst diseaseen_US
dc.titleDevelopment And Evaluation Of An Antigen Detection Test For Hydatid Cyst Diseaseen_US
dc.typeThesisen_US
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