Analytical Method Development And Fundamental Studies On The Separation Of Drugs Using Capillary Electrophoresis
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Date
2011
Authors
Al Azzam, Khaldun Mohammad Mitlaq
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Capillary zone electrophoresis (CZE) methods for the simultaneous separation of the β-blocker drugs (atenolol (AT), chlorthalidone (CH) and amiloride (AM)), using UV and capacitively coupled contactless conductivity detectors (C4D) were developed and validated. Under the adopted conditions, the analytes were separated in less than 4 min and 7 min for the CZE-UV and the CZE-C4D methods, respectively. The CZE-C4D method has slightly inferior sensitivity, but nevertheless, both methods were successfully applied to the determination of the active ingredients in pharmaceutical preparations.
A micellar electrokinetic chromatography (MEKC) method for the simultaneous determination of the antiviral drugs acyclovir (ACV) and valacyclovir (VCV) and their major impurity (guanine) was developed. Under the adopted conditions (BGE of 20 mM citric acid adjusted with 1 M tris solution (pH 2.75) containing 125 mM sodium dodecyl sulphate), and analytes were all separated in about 4 min.
A CZE method for the simultaneous separation of the enantiomers of both ofloxacin and ornidazole using sulfated-β-cyclodextrin (S-β-CD) as chiral selector is also described. Good analysis time (less than 16 min) with resolution of 5.45 and 6.28 for ofloxacin and ornidazole enantiomers, respectively, were achieved using a BGE of 50 mM H3PO4 adjusted with 1 M tris solution; pH 1.85; containing 30 mg mL−1 S-β-CD. Recoveries between 97.1 – 104.0 % were obtained. The computational calculations for the enantiomeric inclusion complexes are also described.
A simple CZE assay stability-indicating method for the determination of modafinil in pharmaceutical formulations has been developed. The method showed not only good precision and accuracy but also good robustness. The LOD and LOQ were 1.2 and 3.5 μg mL−1, respectively. Excipients present in the tablets and degraded products from the different stress conditions did not interfere in the assay.
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Keywords
The separation of drugs , using capillary electrophoresis