Evaluation Of Glutathione Disulphide On Oil Palm Seedlings (Elaeis guineensis) At Nursery Stage
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Date
2017-06
Authors
Wong, Yen Siang
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Publisher
Universiti Sains Malaysia
Abstract
In the oil palm industry, increasing yield productivity without further land expansion is an important priority for big companies and smallholders. Glutathione disulphide (GSSG) has the potential to promote seedling growth and increase palm fruit productivity. This study was conducted to evaluate the effect of GSSG on the growth of oil palm seedlings grown under nursery and greenhouse conditions. The seedlings were tested with two forms of GSSG that were liquid and granules. After 120 days of growth in the greenhouse, seedlings were harvested and determined for dry weight, chlorophyll content and nutrients content. The antioxidant activities of the seedlings were compared with three different assays namely DPPH, FRAP and metal chelating assay. All parameters measured were then statistically analyzed. Genomic DNA was also extracted from the soil of palm oil and 16S rDNA metagenomic sequencing was carried out to determine bacterial abundance between GSSG-treated and control soil. The results revealed that the top biomass and root volume in oil palm seedlings was effectively enhanced up to 108% and 140% respectively due to exogenous application of GSSG solution. Bigger leaves and height increase was observed for GSSG solution-treated seedlings compared to the control. Besides, DPPH scavenging activity was significantly higher in GSSG-treated seedlings (6.36 mg/mL – 8.04 mg/mL) than the control seedlings (10.64 mg/mL). The 16S rDNA metagenomic analysis revealed diversity of bacterial communities in the soil. Both Proteobacteria and Firmicutes accounted for the biggest abundance of between 30% and 60% in the soil for all the treatments observed. The results presented here suggested that GSSG have the potential to be utilized and applied at oil palm nurseries.
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Keywords
Evaluation of glutathione disulphide , on oil palm seedlings at nursery stage