The Relationship Between Protein And Mrna Expression Of Transcription Repressor Dream, C-Fos And Prodynorphin In Modulating Pain Responses Induced By Formalin In The Rat Spinal Cord

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dc.contributor.authorLong, Idris
dc.date.accessioned2019-10-04T02:38:43Z
dc.date.available2019-10-04T02:38:43Z
dc.date.issued2011-07
dc.description.abstractDownstream Regulatory Element Antagonist Modulator (DREAM) protein acts as a transcription repressor for c-Fos and prodynorphin gene which is involved in modulating pain processes. This study was conducted to investigate the relationship between DREAM, c-Fos, prodynorphin protein and mRNA expression through opioid and non-opioid receptors in the modulation of pain responses induced by formalin in the rat spinal cord. Male Sprague Dawley rats weighing between 250-300 g were divided into five major groups. Group 1 consisted of rats treated with pre-emptive administration of ketamine (anaesthetic drug) (5 mg/kg body weight) intraperitoneally (i.p) and given formalin injection (K+F group) (n =36), 30 minutes later. Group 2 consisted of rats treated with pre-emptive administration of norbinaltorphimine dihydrochloride (norBNI) (kappa opioid receptor antagonist) (2 mg/kg body weight) i.p and given formalin injection (N+F group) (n = 36) after 24 hours. Rats in group 3 were treated with pre-emptive administration of both ketamine and norBNI i.p and given formalin injection (NK+F group) (n=36). Rats in group 4 were only treated with normal saline and given formalin injection (F group) (n= 36) while rats in group 5 were not given any treatment and considered as a control group (C group) (n =18). Each experimental group except the control group was further divided into subgroups (n = 6) consisting of rats that were sacrificed at 2 and 4 hours after formalin injection. Fifty μl of formalin 5% (1.85% formaldehyde solution), was subcutaneously injected into the plantar surface of the left hind paw of the rat.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8946
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectProtein And Mrna Expressionen_US
dc.subjectThe Rat Spinal Corden_US
dc.titleThe Relationship Between Protein And Mrna Expression Of Transcription Repressor Dream, C-Fos And Prodynorphin In Modulating Pain Responses Induced By Formalin In The Rat Spinal Corden_US
dc.typeThesisen_US
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