Characterization Of Cal 27 Oral Squamous Carcinoma Cell Line As A Model For Cancer Stem Cell Study
| dc.contributor.author | Chai, Yuan Lin | |
| dc.date.accessioned | 2017-01-20T02:54:02Z | |
| dc.date.available | 2017-01-20T02:54:02Z | |
| dc.date.issued | 2016-09 | |
| dc.description.abstract | The percentage of cancer stem cells (CSCs) is relatively low in tumour bulk population. Therefore, isolation and enrichment steps for CSCs have become routine for research works involving CSC. CAL 27 (ATCC® CRL-2095 TM) is one of the commonly used squamous epithelial carcinoma cell lines derived from human tongue tissue. The present study was conducted with the objective to establish a suitable cell line model for studying CSC development efficiently, by reducing the time consuming isolation and enrichment steps. Experiments were carried out to determine the morphological as well as physiological transformations of CAL 27 cell line, particularly on the expression of mesenchymal stem cell markers, cell doubling time, treatment resistance, phenotype reversibility, invasiveness and metastatic ability. In addition to that, involvement of cell adhesion and epithelial-mesenchymal transition (EMT), together with Wnt signaling pathway were investigated. As for the results, elongation of CAL 27-O cells (CAL 27 cells from 6th passage onwards) from CAL 27-Y cells (CAL 27 cells below 6th passage) were found to be associated with enhanced invasive and metastatic capability, greater resistance to treatment, reversal of phenotype characterizing epithelial cells, besides enhanced proliferation. Moreover, cells expressing mesenchymal stem cell positive marker (CD105) was increased for more than 70% with the transition. Contrasting to this, cells with cell-cell adhesion protein (CD166) was reduced by more than 90%, accompanied by overexpression of vimentin and downregulated expression of E-cadherin for EMT. Besides, the phenotypic transformations were associated with downregulation of Wnt noncanonical planar cell polarity (PCP) pathway in CAL 27-O cells. Hence, the study could be extended in CSC related study, to understand the development of CSCs and also for screening of potential drugs targeting CSCs. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/3517 | |
| dc.subject | The percentage of cancer stem cells (CSCs) is relatively low | en_US |
| dc.subject | in tumour bulk population. | en_US |
| dc.title | Characterization Of Cal 27 Oral Squamous Carcinoma Cell Line As A Model For Cancer Stem Cell Study | en_US |
| dc.type | Thesis | en_US |
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