Mapping Of Transcriptional Start Site And The Identification Of Novel Srna In Mycobacterium tuberculosis H37Rv
| dc.contributor.author | Lee, Li Pin | |
| dc.date.accessioned | 2019-07-15T01:06:08Z | |
| dc.date.available | 2019-07-15T01:06:08Z | |
| dc.date.issued | 2016-09 | |
| dc.description.abstract | The adaptability of Mycobacterium tuberculosis (Mtb) to various environmental changes presented by host immune systems enables its persistence within infected hosts for decades. Various aspects of gene expression regulation in response to environmental stress have been extensively analyzed in Mtb. However, the roles of transcriptional start sites (TSSs) and the small regulatory RNAs (sRNAs) in regulating gene expression within Mtb are given very little attention, particularly under stress condition. This ignites the effort to map TSSs and identify sRNAs involved in different stress environments. In order to map the TSSs in Mtb, cDNA libraries that specifically enriched with primary transcripts were constructed for RNA sequencing, and TSSpredator was integrated for the analysis of TSSs in Mtb monitored in different stress environments. Here, a total of 8,173 TSSs were mapped in six conditions. The majority of TSSs (3,904) were detected within annotated genes (internal TSS), and 1,932 primary TSSs reside within 300 nt upstream of translation start site. 2,861 TSSs correspond to potential antisense sRNAs (antisense TSS). Finally, 215 novel TSSs are located within intergenic regions and represent potentially novel trans-encoded sRNAs. The mapping of TSSs allows identification of alternative transcripts and 5’ untranslated regions (UTR), thus refining the existing annotation of transcriptional landscapes. In addition, mapping of TSSs also manifested an increasing number of leaderless transcripts under stress condition. This suggests the regulatory roles of leaderless transcripts in Mtb’s physiology for stress survival. To detect potential sRNAs in Mtb, cDNA libraries were constructed from size-fractionated total RNA to enrich the small sizes RNAs (<120 nt) for RNA-seq. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/8482 | |
| dc.language.iso | en | en_US |
| dc.publisher | Universiti Sains Malaysia | en_US |
| dc.subject | Mapping of transcriptional start site | en_US |
| dc.subject | the identification of novel sRNA | en_US |
| dc.title | Mapping Of Transcriptional Start Site And The Identification Of Novel Srna In Mycobacterium tuberculosis H37Rv | en_US |
| dc.type | Thesis | en_US |