In Vitro Assessment Of Coagulation Activities In Human Plasma Treated With Ajwa Dates (Phoenix Dactylifera L.) Extracts
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Date
2015-08
Authors
Zakaria, Ainul Mardhiyah
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Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
Arterial and venous thromboses contribute to significant morbidity and mortality rate. Antithrombotic agent is needed for prevention and treatment of thrombosis. Dates (Phoenix dactylifera L.) were reported to have high salicylic acid, a compound responsible for anticoagulant by acting as vitamin K antagonist. Thus, dates may have anticoagulant effect. The present study was design to assess coagulation activities in human plasma treated with Ajwa dates extracts in vitro. Platelet poor plasma (PPP) from twenty-seven donors (n=27) were treated with Ajwa dates ethanol and aqueous extracts at different concentrations (0.1, 0.5 and 1.0 g/mL). Seven groups were formed from each donor: control (normal saline), ET I (0.1 g/mL), ET II (0.5 g/mL), ET III (1.0 g/mL), AQ I (0.1 g/mL), AQ II (0.5 g/mL), AQ III (1.0 g/mL). In vitro coagulation activities of Ajwa dates were assessed by prothrombin time (PT), activated partial thromboplastin time (APTT) and thrombin time (TT). Very significant prolongation of PT, APTT and TT were observed for concentration ET II and ET III. Very significant prolongation of PT and TT were observed for concentration AQ II and AQ III. Significant prolongation of TT was observed in AQ I. Coagulation in extrinsic, intrinsic and final coagulation systems were prolonged at the same ethanol extract concentrations. Extrinsic pathway and final coagulation systems showed prolongation by aqueous extract. Final coagulation system was more affected compare to extrinsic pathway because prolongation at concentration AQ I was observed. In conclusion, Ajwa dates showed anticoagulation effect.
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Keywords
Treatment of thrombosis