Profiling And Unrestricted Identification Of Post-Translational Modifications Of Proteins In The Hevea Brasiliensis Latex

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dc.contributor.authorHabib, Mohd Afiq Hazlami
dc.date.accessioned2018-02-02T07:01:21Z
dc.date.available2018-02-02T07:01:21Z
dc.date.issued2017-04
dc.description.abstractThe natural rubber latex extracted from the bark of Hevea brasiliensis plays various important roles in today’s modern society. After ultracentrifugation, the latex were separated into 3 layers; C-serum, lutoids, and rubber particle. Research showed that a large number of proteins could be found inside these three layers. However, a complete proteome for this important plant is still unavailable and only a handful of post-translational modifications (PTMs) of the proteins have been reported. Protein sequences were translated from the completed draft genome database of H. brasiliensis. The sequences were used to create the annotated protein databases of H. brasiliensis biosynthetic pathways; photosynthesis, latex allergens, rubberwood formation, latex biosynthesis, and disease resistance, which were used for this research. This research was conducted to identify the proteins contained within the latex by way of de novo sequencing from the mass spectra obtained from the 3 layers of the latex. Peptides from these proteins were fragmented using collision-induced dissociation (CID), higher-energy collisional dissociation (HCD) and electron-transfer dissociation (ETD) activation methods. A large number of unique proteins were identified from the natural rubber latex by using the PEAKS software workflow. Unrestrictive identification of PTMs was also conducted without protein enrichment and PEAKS was able to identify potentially important PTMs such as phosphorylation, methylation, acetylation and glycosylation within the proteome of the rubber latex. All PTMs that were identified during database search were crosschecked with the records from the UniProt as well as results from PTM predictions tools. A large percentage of proteins were identified from the H. brasiliensis biosynthetic pathways; photosynthesis, latex allergens, rubberwood formation, latex biosynthesis, and disease resistance databases, which amounts to 100%, 90.5%, 100%, 92.5%, and 63.3% respectively. A total of 1839 and 4038 proteins were identified from AnnHBM and PlantGDB, respectively as well as 133 peptides in 60 proteins from H. brasiliensis protein sequences derived from UniProtKB were identified and predicted to have PTMs. The identification of latex proteins and PTMs from this research could provide significant advantage towards the advancement of rubber industry such as increasing the rubber quality, disease resistance and latex yield of the H. brasiliensis.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/5485
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectProfiling and unrestricted identification of post-translational modificationsen_US
dc.subjectof proteins in the hevea brasiliensis latexen_US
dc.titleProfiling And Unrestricted Identification Of Post-Translational Modifications Of Proteins In The Hevea Brasiliensis Latexen_US
dc.typeThesisen_US
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