Kepatogenan ganoderma boninense pada kelapa sawit dan hubungan biologinya dengan ganoderma spp. daripada perumah palma lain
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Date
2007-04
Authors
Jer Jing, Chan
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Abstract
Pathogenicity tests were carried out on oil palm seedlings and germinated seeds
using monokaryotic and dikaryotic mycelia in order to investigate the role of
dikaryotization in conferring pathogenicity. The results showed that monokaryotic
isolates were non-pathogenic while reconstituted dikaryotic mycelia can initiate
basal stem rot infection. The inoculation method of placing germinated oil palm
seeds in direct contact with rubberwood blocks well-colonized with dikaryotic
mycelia gave faster and consistent results as the disease symptoms started to
appear four months after inoculation.
Mating compatibility tests were carried out using Ganoderma monokaryon tester
strains to study the biological relationship of G.boninense from oil palm and
Ganoderma spp. from other palm hosts. The results showed that G. boninense
crossed with other Ganoderma isolates from oil palm, including G. miniatocinctum
and G. zonatum, as well as isolates from sealing wax palm, MacArthur palm and
coconut. However, G. tornatum did not cross, which showed that it is not
conspesific with all the other Ganoderma isolates from other palm hosts.
Molecular analyses using PCR-RFLP of ITS1-5.8S-ITS2 showed that different
restriction patterns were obtained from isolates from different hosts. UPGMA cluster
analysis of the restriction patterns showed that Ganoderma isolates from various
palms and hard wood (tea and rubber) were clustered separately. Therefore, PCRXV
RFLP analysis of ITS1-5.8S-ITS2 strongly supports mating data in species
delimitation of Ganoderma.
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Keywords
Ganoderma boninense pada kelapa sawit , Ganoderma spp. daripada perumah palma