Biological and chemical characterization of centella asiatica accession (CaFR04)

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dc.contributor.authorAbas, Rafedah
dc.date.accessioned2015-07-30T04:50:44Z
dc.date.available2015-07-30T04:50:44Z
dc.date.issued2007
dc.description.abstractCentella asiatica, locally known as pegaga contains a diverse array of triterpenoid compound that have causal effects in various diseases. The major constituents of pegaga are the four triterpenes, asiatic acid, asiaticoside, madecassic acid and madecassoside. In Malaysia, there are 14 accessions of C.asiatica that have been studied previously and four accessions were identified as potential accessions of pegaga. In the present study, one accession was selected among the potential accessions and named as a CaFR04 to investigate the effect of harvesting period and the fertilizer treatment on the production of triterpenes and the biological activities. The plant samples were extracted with methanol and ethanol-aqueous and analysed by HPLC. A high concentration of triterpenes was obtained in both methanol and ethanolaqueous extract at month two of harvesting and chicken manure as a fertilizer. Asiaticoside was always found at high concentrations but very low concentrations of madecassic acid and asiatic acid were detected. CaFR04 shows high antioxidant activity at each month of harvesting and also from every treatment of fertilizer and control group. Only methanol extract of CaFR04 harvested at month three was active against MCF-7, Vero and WRL-68 cell lines whereas no activity was detected from each treatments of fertilizer and control group. The study can be further clarified by comparing on the level of proteins by using proteomics approach. The protein profile of CaFR04 and the changes due to the response to the different harvesting period and fertilizer treatment were obtained using SDS-PAGE and SELDI-MS analysis. The pattern of the SDS-PAGE gel was similar at each month of harvesting and from each treatment of fertilizer and control group. The SDS-PAGE is typically focused on the 15 - 200 kDa range while SELDI-MS is used for analysis of proteins in particular those of < 40 kDa. The protein peaks obtained from SELDI-MS analysis varied with different ProteinChip® surfaces. The SDS-PAGE gel profile of CaFR04 was compared with other accessions. Different pattern of protein bands was observed. The 31 kDa protein bands found in abundance in CaFR04 were also detected in other accessions, namely CaFR05, CaFR06, CaFR07 and CaFR08 but the 23 kDa protein bands present in CaFR04 were not detected in other accessions. The 31 kDa and 23 kDa protein bands were digested with trypsin and the peptides were analysed by MALDI TOF MS. The 31 kDa protein was identified to match the oxygen-evolving enhancer protein 1 from Pisum sativum (garden pea) but no significant match for 23 kDa protein was found.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1024
dc.language.isoenen_US
dc.subjectChemical characterizationen_US
dc.subjectCentella asiaticaen_US
dc.titleBiological and chemical characterization of centella asiatica accession (CaFR04)en_US
dc.typeThesisen_US
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