Development Of Nano-Formulation Using Pvp/P407 Based On Solid Dispersion From Standardised Ethanolic Extract Of Orthosiphon Stamineus Leaf For Solubility And Oral Bioavailability Improvements

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dc.contributor.authorSaidan, Noor Hafizoh
dc.date.accessioned2019-09-03T01:26:52Z
dc.date.available2019-09-03T01:26:52Z
dc.date.issued2017-08
dc.description.abstractThis study involved the quality assessment of Orthosiphon stamineus raw materials, standardisation of different extracts, development of nano-formulation using macerated ethanolic extract and evaluated for selected biological activities (antioxidant and cytotoxicity). The dried leaves of O. stamineus fulfilled the physicochemical properties, heavy metals and microbial limits to be used for further study. Five different extracts namely methanolic, methanolic (50%), ethanolic, ethanolic (50%) and water were obtained using three different techniques (Soxhlet, reflux and maceration). The spectroscopic (FTIR and UV) and chromatographic (HPLC) analysis were carried out for standardisation of the crude extracts. The results revealed the flavonoids and phenolic acids were the major chemical constituents in O. stamineus extracts. An improved RP-HPLC/DAD method was validated for the quantitative analysis of rosmarinic acid (RA), 3-hydroxy-5, 6, 7, 4-methoxyflavone (TMF), sinensetin (SIN) and eupatorin (EUP) in the crude extracts. This method was applied in the pharmacokinetic and stability studies in the extract and formulated extract. RP-HPLC/DAD method validation including specificity, linearity (R2 ≥ 0.999), precision (intra- and inter-day) and recoveries (90.2-104%). The LOD and LOQ of this selected compounds were in the range of 0.17 ± 0.01 to 0.24 ± 0.03 μg/mL and 0.53 ± 0.03 to 0.73 ± 0.09 μg/mL, respectively. The FTIR fingerprint and HPLC data set were analysed using chemometric tools (PCA and HCA) for its metabolite contents (primary and secondary) and selected activities.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/8771
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectDevelopment of nano-formulation using macerated ethanolic extracten_US
dc.subjectfor selected biological activities antioxidant and cytotoxicityen_US
dc.titleDevelopment Of Nano-Formulation Using Pvp/P407 Based On Solid Dispersion From Standardised Ethanolic Extract Of Orthosiphon Stamineus Leaf For Solubility And Oral Bioavailability Improvementsen_US
dc.typeThesisen_US
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