The Proteome Analysis Of Human Hepatocellular Carcinoma Cell Line Hepg2 In Response To Catharanthus Roseus-Silver Nanoparticles: A Preliminary Study
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Date
2019
Authors
Muniandy, Komala Selve
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
The incidence of human hepatocellular carcinoma is increasing with
alarmingly mortality rates. Despite many other treatments, biosynthesised silver
nanoparticles show remarkable anticancer activity. However, the protein
expression of these C. roseus-AgNPs treated cancer cells remain unexplored.
Therefore, a preliminary study was conducted to identify and analyse the
differentially expressed proteins in hepatocellular carcinoma cell line HepG2
treated with C. roseus-AgNPs. The HepG2 cell line was treated with 12 μg/mL of
C. roseus-AgNPs for 24 hours. Following the treatment, the cells were observed
under the microscope. The proteins extracted from the cells were separated using
the Sodium Dodecyl Sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and
further identified. The molecular weight of the proteins were also identified by
migration distance and cross referred in the Expasy:SIB Bioiformatics Resource
Portal. Significant morphological changes of the treated cells were observed. The
treated HepG2 cell line showed potential up regulated proteins in comparison to
the control. The analysed proteins with approximately 103.0 kDa, 63.0 kDa and
39.0 kDa may be related to carcinogenesis and immunoregulatory activity. The
findings obtained would highlight the potential protein as therapeutic and
prognostic tools particularly for hepatocellular carcinoma.
Description
Keywords
Nanoparticles