Soil Physical Factors Affecting Disease Severity Of Root-Knot Nematode On Tobacco And Morphology And Molecular Identification Of Tropical Nematodes In East Coast Malaysia

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Date
2013
Authors
Ishak, Mohd Nurmuhaimin Adli
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Publisher
Universiti Sains Malaysia
Abstract
In Malaysia, tobacco industry is very crucial in uplifting the socio-economic status of farmer in Kelantan and Terengganu. Root-knot nematodes (Meloidogyne spp.) lower the production and quality of tobacco. In this study, 24 tobaccos cultivation areas were surveyed for root gall disease and species diversity of root- knot nematode. Soil physical and pH analysis were carried out to investigate their correlation to disease severity and number of root-knot nematode number in soil. Isolated Meloidogyne spp. was observed for morphology for genus identification and further analyses via Touchdown Polymerase chain reaction (TD-PCR) for species identification. From 24 cultivation area, 22 showed infestation of root-knot nematode. Tobacco plant infected with root-knot nematode showed root gall, yellowing leaves and stunted growth. One- Way ANOVA analysis showed that soil physical properties, and soil pH affect disease severity and Meloidogyne spp. number in soil. Meloidogyne spp. numbers in soil also affect disease severity. From Pearson correlation analysis, there was significant correlation at 0.01 between Meloidogyne spp. number in soil (r = 0.753), soil pH (r = 0.238), soil moisture (r = 0.203) and soil bulk density (r = 0.227) to disease severity.
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Keywords
Soil Physical Factors Affecting Disease Severity , Root-Knot Nematode On Tobacco And Morphology And Molecular Identification
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