Pemilihan dan propagasi klon elit sentang, azadirachta excelsa L.
| dc.contributor.author | Teik Kooi, Liew | |
| dc.date.accessioned | 2015-09-28T07:22:08Z | |
| dc.date.available | 2015-09-28T07:22:08Z | |
| dc.date.issued | 2000-06 | |
| dc.description.abstract | Sentang, Azadirachta exce/sa L. seeds that were sown 0.5 em below the surface of a container filled with sand showed 90% germination rate after three weeks. Eight elite clones were selected after six months of planting from a population of sentang (n= 150) using a special program created from the PAS CAL language. Immersing the base of five-node apical cuttings of elite sentang clones into 2,500 ppm indole butyric acid (IBA) produced 100% rooting after three weeks of planting. Plants with five-node shoots decapitated induced 100% growth of the first axillary bud, 45% growth of the second axillary bud and 5% growth of the third axillary bud from the cut surface of the stem. Tap root formation were seen in rooted cuttings after three months of planting. In the two stage sterilization experiment, axillary buds that were first sterilized in 25% Clorox® solution for 25 minutes and followed by 20% Clorox® solution for 15 minutes produced 85% sterile explants with 80% survival rate. Axillary buds cultured on solid MS medium + 0.1 mg/1 !-naphthalene acetic acid (NAA) + 1 mg/1·6- bcnzylaminopurine (BA) produced eight shoots per explant. In vitro subculturing of vertically orientated shoots into magenta boxes (polypropylene container, GA 7) containing MS medium + 0.05 mg/1 NAA + 0.75 mg/1 BA produced 10 shoots per explant. Microshoots containing axillary bud and callus of sentang cultured separately on MS medium + 2 mg/1 BA produced 18 adventitious buds and 16 adventitious buds per explant respectively. When in vitro shoots measuring 1.5-2.5 em long were cultured into culture pack containing Y2MS liquid medium + 0.5 mg/1 NAA + 1 mg/1 IBA 1OO% rooting was obtained. Transfer of in vitro plantlets from the shower house . 10 the shade house and subsequent planting in the field successfully acclimatized 80% of the plantlets. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/1236 | |
| dc.language.iso | other | en_US |
| dc.subject | Propagasi klon elit sentang | en_US |
| dc.subject | Azadirachta excelsa L. | en_US |
| dc.title | Pemilihan dan propagasi klon elit sentang, azadirachta excelsa L. | en_US |
| dc.type | Thesis | en_US |
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