The Effect Of Otub1 Overexpression On Human Cervical Cancer Hela
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Date
2015
Authors
Abdul Hamid, Fitrah Mujahidah
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Protein ubiquitination is a highly regulated process that controls multiple physiologically
and pathologically relevant mechanisms involved in tumor development. There is
growing recognition that mutated deubiquitinating enzyme (DUBs) in human cancers
suggesting their roles as oncogenes and tumor suppressors. Previous studies have
identified the DUBs regulate processes associated with cell proliferation and apoptosis,
and as such represent candidate targets for cancer therapeutics. Here we investigated
whether members of the ovarian tumor proteases (OTU) family of DUBs influence the
proliferation in HeLa cells. We intended to study the effect of OTUB1 overexpression in
cervical cancer HeLa and L929 cells lines. Transfection with Lipofectamine™ 3000 was
performed in both cell lines. The transfection efficiency was measured by fluorescence
microscopy. MTT assay was performed to evaluate cell proliferation on HeLa and L929
cells. Statistical analysis was performed by using one way ANOVA test. OTUB1 has
significantly inhibit the proliferation of Hela cells at all incubation times in comparison
to control. In conclusion, overexpression of OTUB1 inhibit the cell proliferation of
HeLa.
Description
Keywords
Tumor