Protein Profiling And Bioactivity Study Of Protein Extracted From Gynura Procumbens (Lour.) Merr.
| dc.contributor.author | CHAW SEN, HEW | |
| dc.date.accessioned | 2016-07-18T04:02:39Z | |
| dc.date.available | 2016-07-18T04:02:39Z | |
| dc.date.issued | 2015-06 | |
| dc.description.abstract | Gynura procumbens (Lour.) Merr. is a traditional medicinal plant for treatments of high cholesterol, high blood pressure, diabetes and cancer. Studies on the small molecule compounds of the plants have shown that the leaves of G. procumbens possess anti-hyperglycaemic, anti-hyperlipidaemic, anticancer and anti-inflammatory activities. Despite the extensive studies on this plant, to date, there is no report on its protein content. Thus, the objectives of the study were to profile the protein content of G. procumbens, whereby the differentially expressed proteins between stem, mature leaf and young leaf of the plant were compared; and the bioactivities of the raw and fractionated protein were evaluated. A gel-based proteomic was established and used to analyse the protein content of the leaves of G. procumbens. Amongst the valuable plant defence proteins detected were peroxidase, thaumatin-like proteins, harpin binding protein 1 and miraculin. Various types of protein categories were identified in the protein extracted from G. procumbens which include the high abundance proteins in mature leaf consisted of proteins involved energy and metabolism, which tally with the role of the mature leaf as the energy generator for the entire plant. The expression of proteins also varied among plants collected from different geographical areas. No antimicrobial activity was detected while poor antioxidant activity was observed in the crude protein extract. On the other hand, the extract exerted LDH cytotoxicity with EC50 value of 760 μg/mL (w/v) after 48 hours treatment on MDA-MB-231, a breast cancer cell line. Active protein fraction SNF11/ 12 obtained from gel filtration fractionation inhibited the growth of MDA-MB- 231 at EC50 value of 3.8 μg/mL (w/v). The mRNA expressions of proliferation markers, Ki67 and PCNA were reduced significantly (p<0.05) in the MDA-MB-23 cells treated with SN-F11/12. The expression of an invasion marker, CCL2, was also reduced in the treated MDA-MB-231 cells. Twelve proteins were identified in the SN-F11/12 by mass spectrometry analysis. Further investigation on SN-F11/12 is needed to evaluate its usefulness as a chemotherapeutic drug. This study showed that G. procumbens has some commercial valuable proteins and the protein fraction, SNF11/ 12 demostrated anti-cancer property. The proteomes of stem, mature leaf and young leaf were successfully developed and provided a platform for further research. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/2283 | |
| dc.subject | Protein Profiling And Bioactivity Study Of Protein Extracted | en_US |
| dc.subject | From Gynura Procumbens (Lour.) Merr | en_US |
| dc.title | Protein Profiling And Bioactivity Study Of Protein Extracted From Gynura Procumbens (Lour.) Merr. | en_US |
| dc.type | Thesis | en_US |
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