Influence of rain, malathion, sunlit and shaded habitats on the duration of development and molecular identification of chrysomya megacephala (fabricius) (diptera: calliphoridae) in Kelantan, Malaysia
| dc.contributor.author | Mahat, Naji Arafat (Haji) | |
| dc.date.accessioned | 2015-07-30T02:18:22Z | |
| dc.date.available | 2015-07-30T02:18:22Z | |
| dc.date.issued | 2010 | |
| dc.description.abstract | Chrysomya megacephala (Fabricius) is the earliest and the most prevalent necrophagous species infesting dead bodies in Malaysia and thus, may provide the best estimate of minimum postmortem interval (PMI). In Malaysia, heavy and/or incessant rain is commonplace occurrence and malathion is the most prevalent poison used for committing suicide. In this research, the independent and combined influences of rain and presence of malathion in whole rabbit carcasses decomposing in sunlit and shaded habitats on oviposition and development of Ch. megacephala were studied over a period of 1-year in Kelantan, Malaysia for providing baseline data for estimating PMI. Base sequence of CO 1 gene was' used for species identification an'd the influence o(malathion on the sequence of CO I gene of Ch. megacephala was determined. The possible use of larvae and pupae of Ch. megacephala for detecting malathion for diagnosing the cause of death especially when visceral organs have liquified was also investigated. In general, rain, depending on its intensity, delayed initial oviposition by 1-2 days and prolonged the pupation period by 1-3 days. The presence of malathion in the carcasses delayed initial oviposition by l-3 days and prolonged the pupation period by 2-3 days. Presence of rain and malathion cumulatively prolonged pupation period that extended to a maximum of 8 days. It was found that malathion did not impede the accuracy of species identification while using the base sequence of COl gene fragment amplified by C1-J-2495/Cl-N-2800 primers. The detection of malathion in larvae and pupae per se may prove useful in diagnosing the cause of death, since malathion is a poison as against the other drugs that are abused. These findings deserve consideration while estimating PMI relating to decomposed bodies recovered in Malaysia during both the less rainy and rainy months of a year as well as when malathion poisoning is suspected. | en_US |
| dc.identifier.uri | http://hdl.handle.net/123456789/914 | |
| dc.language.iso | en | en_US |
| dc.subject | Shaded habitats | en_US |
| dc.subject | Chrysomya megacephala | en_US |
| dc.subject | Calliphoridae | en_US |
| dc.title | Influence of rain, malathion, sunlit and shaded habitats on the duration of development and molecular identification of chrysomya megacephala (fabricius) (diptera: calliphoridae) in Kelantan, Malaysia | en_US |
| dc.type | Thesis | en_US |
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