Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines

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dc.contributor.authorAlaa Taha Yasir Al Kanan
dc.date.accessioned2021-01-18T08:47:17Z
dc.date.available2021-01-18T08:47:17Z
dc.date.issued2019
dc.description.abstractBackground: Breast cancer is the most common cancer affected women and the incidence rate is increasing yearly throughout the world. Tamoxifen is the first-line chemotherapeutic drug used in treating breast cancer patients. However, gynaecological complications and drug resistance are among the major drawback effects of tamoxifen, particularly at 10-15 years of post-treatment. Thereafter, breast cancer recurrence may occur which also contribute to the major causes of breast cancer-related deaths. Garcinia hombroniana (GH) (seashore mangosteen) is a plant that has been reported to possess good cytotoxic effect against the growth of various human cancer cell lines, including the MCF-7. For this reason, the aim of this study was to further investigate the anti-proliferative and apoptotic effects of the essential oil extracted from the leaves of GH against two different types of breast cancer cell lines, MCF-7 and MCF-7/TAMR-1. Methods: The essential oil was extracted by hydrodistillation process from the leaves of GH. The anti-proliferative effects of the essential oil against MCF-7 and MCF-7/TAMR-1 cancer cell lines were determined using MTT assay and measured by a microplate reader. The mechanism of cell death was determined using Annexin V-FITC/propidium iodide staining assay and quantitatively measured by flow cytometry. The human non-cancerous breast cell line, MCF-10A was also included in both assays as comparative control cells to the MCF-7 and MCF-7/TAMR-1 cells. Results: The results showed that the GH essential oil exhibited anti-proliferative effect against the growth of both MCF-7 and MCF-7/TAMR-1 cells following dose- and time-dependent manners, with an IC50 of 35.22 μg/mL and 17.67 μg/mL, respectively.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/10892
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectPlant extractsen_US
dc.subjectBreast neoplasmsen_US
dc.titleMechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Linesen_US
dc.typeThesisen_US
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