Application Of The Polyhydroxyalkanoate Synthase Of Chromobacterium Sp. Usm2 For The Production Of Biodegradable Plastics

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dc.contributor.authorBhubalan, Kesaven
dc.date.accessioned2018-11-02T03:45:12Z
dc.date.available2018-11-02T03:45:12Z
dc.date.issued2010-05
dc.description.abstractA local isolate, Chromobacterium sp. USM2 was found to synthesize polyhydroxyalkanoate (PHA) with very high molar fractions of 3-hydroxyvalerate (3HV) monomer under certain culture conditions. The PHA synthase gene of this isolate was cloned and expressed in a mutant Cupriavidus necator PHB−4. Interestingly, the synthase was able to incorporate 3-hydroxyhexanoate (3HHx) monomer when this transformant was cultured with palm oil as the sole carbon source. In this study, the PHA synthase of Chromobacterium sp. USM2 (PhaCCs) was characterized via in vivo and in vitro assays using Escherichia coli JM109 to better understand its ability to synthesize PHA. Results from the in vitro assays showed that the expression level of PhaCCs was higher compared to the PHA synthase of C. necator H16 (PhaCCn) which was used as a positive control. This phenomenon was clearly exhibited in the SDSPAGE and Western blot analyses. The total enzyme activity of PhaCCs (2132 U/g) was nearly 8-fold higher compared to that of PhaCCn (253 U/g). These findings were further supported by the results obtained from in vivo assay whereby the E. coli transformant was able to produce 76 ± 2 wt% of poly(3-hydroxybutyrate) homopolymer within 24 h of cultivation with glucose.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/6958
dc.language.isoenen_US
dc.publisherUniversiti Sains Malaysiaen_US
dc.subjectPolyhydroxyalkanoate Synthaseen_US
dc.subjectChromobacterium Sp. Usm2en_US
dc.titleApplication Of The Polyhydroxyalkanoate Synthase Of Chromobacterium Sp. Usm2 For The Production Of Biodegradable Plasticsen_US
dc.typeThesisen_US
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