Diversity, mycotoxin profiles and mating populations of fusarium species associated with non-agricultural grasses in Peninsular Malaysia

Simple item page
dc.contributor.authorIsmail, Nor Azliza binti
dc.date.accessioned2015-08-25T07:18:25Z
dc.date.available2015-08-25T07:18:25Z
dc.date.issued2008-10
dc.description.abstractFusarium is a well-established pathogenic and toxigenic genus of imperfect fungi that cause significant losses to several economically important crops and mycotoxicoses all over the world including Malaysia. A study of Fusarium species on non-agricultural grasses was carried out in Peninsular Malaysia to determine the diversity of Fusarium species based on their morphological characters, mycotoxin profiles and mating populations. A total of 304 isolates comprising 11 species of Fusarium were recovered from 67 fresh and air-dried grass samples from 24 sampling sites in 1 0 states. The isolates were identified as F. semitectum (96 isolates, 31.6%), F. so/ani (58 isolates, 19.1%), F. equiseti (43, 14.1%), F. compactum (31 isolates, 1 0.2%), F. oxysporum (25, 8.2%), F. proliferatum (20 isolates, 6.6%}, F. sacchari (10 isolates, 3.3%), F. subglutinans (10 isolates, 3.3%), F. chlamydosporum (7 isolates, 2.3%), F. nelsonii (1 isolate, 0.3%), F. lateritium (1 isolate, 0.3%), and two unidentified species that were Fusarium sp. 1 (1 isolate, 0.3%) and Fusarium sp. 2 (1 isolate, 0.3%). Four mycotoxins i.e. moniliformin (MON), fumonisin 81 (FUMB1), zearalenone (ZEN), and beauvericin (BEA) were detected in sterilized corn grits inoculated with conidial suspensions of 31 strains of Fusarium species. BEA showed the highest frequency of toxin detected ( 14 strains), followed by MON (9 strains), ZEN (3 strains), and FUMB1 (1 strain). The concentrations of BEA and ZEN were from 0.3 - 37.8 j..ll/g and 0.14 - 4.0 j.Jl/g, respectively. Bioassays on A. salina larvae showed high mortality rates in extracts containing MON (F. proliferatum, D3474&N; F. chlamydosporum, P3590&N), FUMB1 (F. proliferatum, D3474&N) and BEA (F. semitectum, J3526&N; F. oxysporum, P3610&N; F. proliferatum, 03474&N; Fusarium sp. 1, P3591&N). All extracts with ZEN exhibited less toxicity towards the larvae. After sexual crosses with standard tester strains, 29 field strains were found to be fertile and produced ascospores in the perithecia while the remaining 11 strains were infertile. Three mating populations (MPs) were recognized i.e. MP-8 (G. sacchari; 10 strains), MPD (G. intermedia; 17 strains) and MP-E (G. subg/utinans; 2 strains). All strains of G. sacchari carried MATB-2 allele, G. intermedia carried both MATD-1 (14 strains) and MA TD-2 (3 strains) alleles whereas G. subglutinans carried MATE-2 allele. This is the first report on the occurrence of Fusarium species on non-agricultural grasses in Peninsular Malaysia.en_US
dc.identifier.urihttp://hdl.handle.net/123456789/1109
dc.language.isoenen_US
dc.subjectMycotoxin profiles and mating populationsen_US
dc.subjectFusarium speciesen_US
dc.subjectNon-agricultural grassesen_US
dc.titleDiversity, mycotoxin profiles and mating populations of fusarium species associated with non-agricultural grasses in Peninsular Malaysiaen_US
dc.typeThesisen_US
Files
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Pusat Pengajian Sains Kajihayat - Tesis