Cytotoxic Effect, Anti-Proliferative And Cell Death Mechanism Of Latex C-Serum And Sub-Fractions Using Cell-Based Assay
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Date
2018-03
Authors
Lam, Kit Lay
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Journal ISSN
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Publisher
Universiti Sains Malaysia
Abstract
Latex from Hevea brasiliensis, the natural rubber tree, has never been reported for folk medicinal use although highly regarded as an economic importance. Latex was thought to play a biological role in the plant defense system against microorganisms and insects. Due to scarce information available on the studies of its biological effects on mammalian system, cell viability assay using MTT, real-time PCR, cell cycle analysis and protein profiler analysis were used to evaluate the anticancer effects of and cell death pathway induced by whole C-serum (WC) and its fractions against the cancer-origin cell lines. Further fractions of the sera used in this study were prepared via dialysis and pre-heat treatment. The resulted four fractions from the whole C-serum were as follows: DCP = dialyzed C-serum precipitant; DCS = dialyzed C-serum supernatant; BCP = boiled C-serum precipitant and BCS = boiled C-serum supernatant. MTT assay revealed that DCS and DCP treatments specifically target HepG2 cells with IC50 values of 0.17 μg/mL ± 0.02 and 1.81 μg/mL ± 0.08 respectively. MDA-MB231 cells were found to be susceptible to DCS and DCP treatments, with IC50 values at 2.00 μg/mL ± 0.04 and 5.49 μg/mL ± 0.01 respectively. DCS was proven to be more effective compared to DCP in conferring specific antiproliferation effects against the tested cancer-origin cell lines. The antiproliferation activity of the pre-heated BCP and BCS was significantly reduced and the results suggested that the active component(s) were heat-labile.
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Keywords
Cytotoxic effect, anti-proliferative and cell death mechanism , of latex c-serum and sub-fractions