Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4

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Date
2009
Authors
Chee, Mei Sing
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Publisher
Universiti Sains Malaysia
Abstract
Detection of hydrocarbon-degrading enzymes activities in microorganisms capable of utilizing crude oil as a carbon source were done using potential oil-degraders isolated from soil and water samples collected from water and oil-contaminated areas in Penang. Seven potential isolates categorized as Group I bacteria were selected based on the criteria that they displayed good growth on solid crude oil medium using 6 different types of crude oil which were named as Tapis, Khefji Dubai, Bunga Kekwa, Angsi, Dulang, and Penara. These crude oil media were prepared without the addition of surfactant. Out of seven isolates, oil-degrading bacterium designated as FETL C4 displayed good growth on different types of crude oil media prepared. Biochemical test results identified this isolate as Acinetobacter sp. Further confirmation was done using 16S rRNA gene analysis and Remel Rapid Identification Kit. Aldehyde dehydrogenase (ALDH) activity was detected during the cultivation of this isolate when grown in minimal salt medium containing 1% (w/v) Tapis crude as a carbon source.
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Keywords
Crude Aldehyde Dehydrogenase On The Degradation , Acinetobacter Baumannii Fetl C4
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