Effect Of Crude Aldehyde Dehydrogenase On The Degradation Of Crude Oil By A Local Isolate, Acinetobacter Baumannii Fetl C4
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Date
2009
Authors
Chee, Mei Sing
Journal Title
Journal ISSN
Volume Title
Publisher
Universiti Sains Malaysia
Abstract
Detection of hydrocarbon-degrading enzymes activities in microorganisms capable
of utilizing crude oil as a carbon source were done using potential oil-degraders
isolated from soil and water samples collected from water and oil-contaminated
areas in Penang. Seven potential isolates categorized as Group I bacteria were
selected based on the criteria that they displayed good growth on solid crude oil
medium using 6 different types of crude oil which were named as Tapis, Khefji
Dubai, Bunga Kekwa, Angsi, Dulang, and Penara. These crude oil media were
prepared without the addition of surfactant. Out of seven isolates, oil-degrading
bacterium designated as FETL C4 displayed good growth on different types of crude
oil media prepared. Biochemical test results identified this isolate as Acinetobacter
sp. Further confirmation was done using 16S rRNA gene analysis and Remel Rapid
Identification Kit. Aldehyde dehydrogenase (ALDH) activity was detected during
the cultivation of this isolate when grown in minimal salt medium containing 1%
(w/v) Tapis crude as a carbon source.
Description
Keywords
Crude Aldehyde Dehydrogenase On The Degradation , Acinetobacter Baumannii Fetl C4